Scholarlink

<p>The investigation was done to determine toxicity tolerance level of ammonia in zebrafish (<i>Danio rerio</i>) as the fresh water fish model and find out the indicative changes in behavioural patterns to identify ammoniacal toxicant induced stress. For this, adult age-matched zebrafish were exposed to 0.1&#xa0;mg/L to 5&#xa0;mg/L conc. of NH<sub>3</sub> consecutively for 96&#xa0;h. When measured under bioavailable NH<sub>3</sub> fixed for the experimental groups, LC<sub>50</sub> value was 0.89&#xa0;mg NH<sub>3</sub>/l under repetitive exposure which was confirmed by Kaplan–Meier curve showing cumulative survival &gt; 0.6 at 72&#xa0;h but &lt; 0.4 at 96&#xa0;h for 1&#xa0;mg/L NH<sub>3</sub> repetitive exposure. Quantitative behavioural analysis showed increasing NH<sub>3</sub> conc. and consecutive exposure decreased playing behaviour from ~ 60% to 30% for sub-acute toxicity and to 5% and less for acute toxicity of NH<sub>3</sub>, whereas increased stress movement from 15–20% to 25–35% for sub-acute and &gt; 90% for acute toxicity. Other significant shifts of behaviour identified that playing, feeding, aggression and stress movements are the key indicative behaviours under ammoniacal stress. Continued contamination of unionized ammonia was found less tolerant to fish and acute to sub-acute levels were reflected though shift of specific behavioural attributes. </p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Ammonia toxicity tolerance and behavioural response in Zebrafish

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年6月30日

查看全文
  • Nabajit Mondal,
  • Jaspreet Kaur,
  • Anirban Ghosh

摘要

The investigation was done to determine toxicity tolerance level of ammonia in zebrafish (Danio rerio) as the fresh water fish model and find out the indicative changes in behavioural patterns to identify ammoniacal toxicant induced stress. For this, adult age-matched zebrafish were exposed to 0.1 mg/L to 5 mg/L conc. of NH3 consecutively for 96 h. When measured under bioavailable NH3 fixed for the experimental groups, LC50 value was 0.89 mg NH3/l under repetitive exposure which was confirmed by Kaplan–Meier curve showing cumulative survival > 0.6 at 72 h but < 0.4 at 96 h for 1 mg/L NH3 repetitive exposure. Quantitative behavioural analysis showed increasing NH3 conc. and consecutive exposure decreased playing behaviour from ~ 60% to 30% for sub-acute toxicity and to 5% and less for acute toxicity of NH3, whereas increased stress movement from 15–20% to 25–35% for sub-acute and > 90% for acute toxicity. Other significant shifts of behaviour identified that playing, feeding, aggression and stress movements are the key indicative behaviours under ammoniacal stress. Continued contamination of unionized ammonia was found less tolerant to fish and acute to sub-acute levels were reflected though shift of specific behavioural attributes.