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<p>The X- and Y-chromosome bearing spermatozoa (X- and Y-sperm) differ in their DNA content, and this difference may be responsible for the differential expression of certain genes. The present study aimed to validate the differential gene expression (DGE) in X- and Y-sperm of <i>Bos indicus</i> bulls to investigate sex-specific differences. Based on the transcriptome dataset, 21 genes were validated by qPCR for their uniqueness/relative expression. Eleven genes in Y-sperm (TA1CL, CPA1, RAB6A, RAB27A, SPATA5L1, SPATS1, TEKT3, TUBB1, TG2C, VDAC1, and SPATA6L) and ten genes in X-sperm (8G2, ADD3, BD, EXOSC1, H2A, DNAJC9, ITPR1P, LEPROTL1, LRRTM3, and SPADH2) are associated with spermatogenesis, motility, capacitation, and fertilization. The qPCR analysis revealed upregulation of nine genes in Y-sperm and eight genes in X-sperm (p &lt; 0.01, p &lt; 0.05), in accordance with the transcriptome dataset. The genes upregulated in Y-sperm are associated with sperm motility and those upregulated in X-sperm with survivability under stress conditions.</p>

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Differentially expressed genes in X- and Y-spermatozoa of Bos indicus bulls

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年11月10日

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  • Dhanu Kumar Murasing,
  • Sofi Imran Ul Umar,
  • Soumen Naskar,
  • Shashi Kumar,
  • Vijai Pal Bhadana,
  • Shiv Prasad,
  • Sujay Rakshit,
  • Dinesh Pandey,
  • A. K. Verma,
  • Mridula Sharma

摘要

The X- and Y-chromosome bearing spermatozoa (X- and Y-sperm) differ in their DNA content, and this difference may be responsible for the differential expression of certain genes. The present study aimed to validate the differential gene expression (DGE) in X- and Y-sperm of Bos indicus bulls to investigate sex-specific differences. Based on the transcriptome dataset, 21 genes were validated by qPCR for their uniqueness/relative expression. Eleven genes in Y-sperm (TA1CL, CPA1, RAB6A, RAB27A, SPATA5L1, SPATS1, TEKT3, TUBB1, TG2C, VDAC1, and SPATA6L) and ten genes in X-sperm (8G2, ADD3, BD, EXOSC1, H2A, DNAJC9, ITPR1P, LEPROTL1, LRRTM3, and SPADH2) are associated with spermatogenesis, motility, capacitation, and fertilization. The qPCR analysis revealed upregulation of nine genes in Y-sperm and eight genes in X-sperm (p < 0.01, p < 0.05), in accordance with the transcriptome dataset. The genes upregulated in Y-sperm are associated with sperm motility and those upregulated in X-sperm with survivability under stress conditions.