<p>Mulberry (<i>Morus alba</i> L.) cultivation supports the livelihood of millions of rural people in eastern India. Excessive use of agrochemicals and inadequate awareness of eco-friendly farming impede environmental sustainability and silk quality. The present study assessed the knowledge, attitudes, and practices of 222 seri-farmers across three blocks of Murshidabad district, West Bengal, India and evaluated the impact of structured training on knowledge and adoption of recommended practices. Seri-farmers were categorized as trained (beneficiaries, <i>n</i> = 131) and untrained (non-beneficiaries, <i>n</i> = 91). Twenty key cultivation and post-cocoon parameters were evaluated using the mean index of knowledge, mean index of adoption, and Impact Index. Improvements were observed in field preparation, sapling cutting treatment, scientific rearing and silk extraction. Nevertheless, improper pesticide use continued due to the influential presence of agro-chemical vendors. Trained seri-farmers showed significantly higher knowledge (94.12 ± 6.35) than the untrained (74.33 ± 8.21; <i>t</i> = 6.84, <i>p</i> &lt; 0.001). Likewise, adoption levels were also better among trained seri-farmers (71.31 ± 9.42) in comparison to untrained (53.00 ± 10.18; <i>t</i> = 5.27, <i>p</i> &lt; 0.001). Additionally, the impact index (19.05%) demonstrated substantial training effectiveness among the trained seri-farmers. These findings suggested that structured sericultural extension programs improve technical knowledge and adoption of sustainable sericultural practices. Thus, strengthening training programmes and improving institutional support are vital to advance economically resilient and environmentally responsible sericulture systems.</p>