Scholarlink

<p>This study investigates the effects of various plant growth regulators on shoot and root development from shoot tip explants of <i>Citrus reticulata</i> cultured in Murashige and Skoog (MS) medium. The combined application of auxin (2,4-dichlorophenoxyacetic acid, 2,4-D) and cytokinin (6-benzylaminopurine, BAP) significantly influenced regeneration. The highest shoot induction rate (60%) and longest shoot length (8.00&#xa0;cm) were achieved with 5.0&#xa0;mg/L 2,4-D and 1.0&#xa0;mg/L BAP, with visible shoot emergence as early as 8 days post-inoculation. Root induction was also optimal at the same concentration, resulting in 50% rooting, an average of 6 roots per explant, and a maximum root length of 5.00&#xa0;cm. Separate evaluations of BAP and naphthaleneacetic acid (NAA) showed enhanced rooting responses at 1.00&#xa0;mg/L BAP (70% rooting) and improved shoot and root growth with 1.46&#xa0;mg/L NAA (30% rooting). Contamination rates remained low throughout the culture stages—8% during multiplication, 5% during shooting, and 3% during rooting—indicating reliable aseptic handling. During acclimatization, plantlets transferred to a sand:soil:compost (1:1:1) mixture exhibited the highest survival rate (90%) and robust growth. These results highlight the effectiveness of specific PGR combinations in promoting in vitro propagation and establish a reliable protocol for the large-scale regeneration and acclimatization of <i>C. reticulata</i>.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Optimizing micropropagation of Kinnow mandarin (Citrus reticulata Blanco) from shoot tip nodal segments

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年8月25日

查看全文
  • Abdiselam Mohammed,
  • Zekeria Yusuf,
  • Yohannes Petros

摘要

This study investigates the effects of various plant growth regulators on shoot and root development from shoot tip explants of Citrus reticulata cultured in Murashige and Skoog (MS) medium. The combined application of auxin (2,4-dichlorophenoxyacetic acid, 2,4-D) and cytokinin (6-benzylaminopurine, BAP) significantly influenced regeneration. The highest shoot induction rate (60%) and longest shoot length (8.00 cm) were achieved with 5.0 mg/L 2,4-D and 1.0 mg/L BAP, with visible shoot emergence as early as 8 days post-inoculation. Root induction was also optimal at the same concentration, resulting in 50% rooting, an average of 6 roots per explant, and a maximum root length of 5.00 cm. Separate evaluations of BAP and naphthaleneacetic acid (NAA) showed enhanced rooting responses at 1.00 mg/L BAP (70% rooting) and improved shoot and root growth with 1.46 mg/L NAA (30% rooting). Contamination rates remained low throughout the culture stages—8% during multiplication, 5% during shooting, and 3% during rooting—indicating reliable aseptic handling. During acclimatization, plantlets transferred to a sand:soil:compost (1:1:1) mixture exhibited the highest survival rate (90%) and robust growth. These results highlight the effectiveness of specific PGR combinations in promoting in vitro propagation and establish a reliable protocol for the large-scale regeneration and acclimatization of C. reticulata.