<p>In the recent world of uncertain climatic alteration, heat stress is a major alarming issue particularly for the agricultural production of winter crops such as wheat. There is still a need of heat tolerant high yielding wheat varieties for sustainable wheat production. Evaluation of diverse germplasm under heat stress can be a significant strategy to find superior wheat genotypes which can be utilized to develop heat tolerant wheat variety. This study evaluated fourteen wheat genotypes including eleven advanced lines, two susceptible checks and one tolerant check under heat stress focusing on the key morpho-physiological traits like normalized difference vegetation index, leaf chlorophyll content, canopy temperature depression, plant height, days to heading, days to maturity, number of spikelets per spike, number of grains per spike, thousand grain weight and grain yield per plot. Principal component analysis efficiently summarized the complex interplay of these traits categorizing the major traits that explained the most significant variation within the collected data. Correlation between specific traits and grain yield indicated the potential of the traits as selection criteria for heat tolerance. Based on all these morpho-physiological traits, this study successfully identified wheat genotypes BAW 1318 and BAW 1290 exhibiting superior performance under heat stress. These genotypes consistently displayed better stability in physiological as well as yield and yield contributing traits compared to other genotypes. So, these genotypes can be recommended to use in further breeding program and the studied traits efficiently be evaluated for enhancing heat tolerance.</p>