Scholarlink

<p>Oil spills are a persistent threat to marine ecosystems, particularly in regions with high shipping traffic such as Ghana’s coastline. Conventional crude oil cleanup methods often generate toxic by-products; making bioremediation a preferred alternative. This study assessed bioaugmented crude oil degradation under biostimulated and non-biostimulated field conditions. <i>Alcanivorax xenomutans</i> and <i>Acinetobacter junii</i> isolated from pristine Ghanaian seawater were applied singly and in a 1:1 combination under natural environmental conditions and in duplicates. Extraction of DNA from bacterial cultures was done using a Zymo DNA extraction kit, followed by genomic sequencing using an Oxford Nanopore Sequencer. Microcosm experiments were conducted on nutrient amended and unamended setups for 45&#xa0;days. In the unamended tests, <i>Acinetobacter junii</i> exhibited degradation efficiencies of 20.68%, 33.33%, and 42.53% on days 15, 30, and 45, respectively. In contrast, <i>Alcanivorax xenomutans</i> achieved degradation efficiencies of 9.54%, 24.14%, and 44.83% on days 15, 30, and 45, respectively. In the amended degradation tests, <i>Acinetobacter junii</i> recorded efficiencies of 33.33%, 47.13%, and 63.22%, while <i>Alcanivorax xenomutans</i> achieved 23.0%, 36.78%, and 49.43% on days 15, 30, and 45, respectively. When both bacteria were applied in combination, the degradation efficiencies were 28.74%, 44.83%, and 48.28% under unamended conditions, and 39.08%, 56.32%, and 67.82% under amended conditions. Bacterial treatments with NPK-amendment produced the highest degradation efficiencies among both the individual and consortium applications. This study proved that a 1:1 mixture of n<i>utrient-amended Alcanivorax xenomutans</i> and <i>Acinetobacter junii</i> showed significant degradation after 30&#xa0;days, indicating their potential for crude oil spill remediation.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Field clean-up of crude oil spills on the coast of Ghana using Alcanivorax xenomutans Acinetobacter junii

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年2月8日

查看全文
  • Mark Kwasi Sarfo,
  • Samuel Fosu Gyasi,
  • Gustav Kudjoe Gbeddy,
  • Harriet Danso-Abbeam,
  • Alfred Kwablah Anim,
  • Andrew Sarkodie Appiah

摘要

Oil spills are a persistent threat to marine ecosystems, particularly in regions with high shipping traffic such as Ghana’s coastline. Conventional crude oil cleanup methods often generate toxic by-products; making bioremediation a preferred alternative. This study assessed bioaugmented crude oil degradation under biostimulated and non-biostimulated field conditions. Alcanivorax xenomutans and Acinetobacter junii isolated from pristine Ghanaian seawater were applied singly and in a 1:1 combination under natural environmental conditions and in duplicates. Extraction of DNA from bacterial cultures was done using a Zymo DNA extraction kit, followed by genomic sequencing using an Oxford Nanopore Sequencer. Microcosm experiments were conducted on nutrient amended and unamended setups for 45 days. In the unamended tests, Acinetobacter junii exhibited degradation efficiencies of 20.68%, 33.33%, and 42.53% on days 15, 30, and 45, respectively. In contrast, Alcanivorax xenomutans achieved degradation efficiencies of 9.54%, 24.14%, and 44.83% on days 15, 30, and 45, respectively. In the amended degradation tests, Acinetobacter junii recorded efficiencies of 33.33%, 47.13%, and 63.22%, while Alcanivorax xenomutans achieved 23.0%, 36.78%, and 49.43% on days 15, 30, and 45, respectively. When both bacteria were applied in combination, the degradation efficiencies were 28.74%, 44.83%, and 48.28% under unamended conditions, and 39.08%, 56.32%, and 67.82% under amended conditions. Bacterial treatments with NPK-amendment produced the highest degradation efficiencies among both the individual and consortium applications. This study proved that a 1:1 mixture of nutrient-amended Alcanivorax xenomutans and Acinetobacter junii showed significant degradation after 30 days, indicating their potential for crude oil spill remediation.