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<p>Biological treatment systems using microbes are more efficient and environmentally friendly compared to conventional technologies for heavy metal removal. In this study, heavy metal- resistant bacterial isolates were identified, and their heavy metal sequestration potential was evaluated in nutrient broth media supplemented with heavy metals and in real wastewater. Out of 65 isolates, Staphylococcus xylosus W1Cr and Staphylococcus xylosus W12Pb were identified based on their cultural, morphological, and molecular characteristics. Genomic DNA extraction and PCR amplification of the 16S rRNA gene were conducted using universal primers, and amplified products were sequenced to confirm species identity. Their accession numbers, PP14113 and PP14115, were available under GenBank as nucleotide information, respectively. Both isolates showed optimal growth at 30&#xa0;°C and pH 8 and exhibited considerable resistance to chromium, cadmium, lead, and zinc. The highest minimum inhibitory concentrations were found to be 4500 and 5500&#xa0;ppm against zinc metals for S. xylosus-W1Cr and W12Pb isolates, respectively. They were capable of achieving high removal of metal both in nutrient broth medium and in real wastewater samples. Most notably, in actual wastewater obtained from the Little Akaki River, individual strains removed as much as 99% of heavy metals, while a co-culture of both strains achieved virtually complete removal (99.98% Pb, 99.79% Zn). The observations suggest the great potential for bioremediation of S. xylosus strains, especially when used in consortium, offering a cost-effective, viable option for the treatment of heavy metal-contaminated water in the developing nations.</p>

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Bioremediation of heavy metals using Staphylococcus xylosus isolates from the little Akaki river, Addis Ababa, Ethiopia

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年7月30日

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  • Smegnew Melese,
  • Bayable Atinafu,
  • Getnet Belay,
  • Addis Simachew

摘要

Biological treatment systems using microbes are more efficient and environmentally friendly compared to conventional technologies for heavy metal removal. In this study, heavy metal- resistant bacterial isolates were identified, and their heavy metal sequestration potential was evaluated in nutrient broth media supplemented with heavy metals and in real wastewater. Out of 65 isolates, Staphylococcus xylosus W1Cr and Staphylococcus xylosus W12Pb were identified based on their cultural, morphological, and molecular characteristics. Genomic DNA extraction and PCR amplification of the 16S rRNA gene were conducted using universal primers, and amplified products were sequenced to confirm species identity. Their accession numbers, PP14113 and PP14115, were available under GenBank as nucleotide information, respectively. Both isolates showed optimal growth at 30 °C and pH 8 and exhibited considerable resistance to chromium, cadmium, lead, and zinc. The highest minimum inhibitory concentrations were found to be 4500 and 5500 ppm against zinc metals for S. xylosus-W1Cr and W12Pb isolates, respectively. They were capable of achieving high removal of metal both in nutrient broth medium and in real wastewater samples. Most notably, in actual wastewater obtained from the Little Akaki River, individual strains removed as much as 99% of heavy metals, while a co-culture of both strains achieved virtually complete removal (99.98% Pb, 99.79% Zn). The observations suggest the great potential for bioremediation of S. xylosus strains, especially when used in consortium, offering a cost-effective, viable option for the treatment of heavy metal-contaminated water in the developing nations.