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<p>Extracellular vesicles (EVs) have emerged as promising biomarkers for liquid biopsies because of their responsiveness to various physiological conditions. However, their dynamic physical properties pose significant analytical challenges, which necessitates the development of novel detection methods. In this study, a portable surface-functionalized power-free microchip (SF-PF microchip) was developed that enables the detection of EVs without external pumps. In particular, the SF-PF microchip specifically detected EVs derived from metastatic breast cancer cells with a limit of detection of 5.0 × 10<sup>10</sup> particles/mL. Moreover, the SF-PF microchip required only 2.0 µL of sample volume and completed the detection within approximately 20&#xa0;min, offering a rapid and convenient approach for EV analysis. Furthermore, the SF-PF microchip successfully detected EVs in serum samples, which highlights its potential for clinical application in liquid biopsies.</p> Graphical abstract <p></p>

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Development of surface-functionalized power-free microchip for breast cancer cell-derived extracellular vesicle detection

  • 期刊论文

  • 发表日期：

    2025年11月20日

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  • Ryo Ishihara,
  • Ryo Matsui,
  • Tadaaki Nakajima,
  • Hinako Yokohari,
  • Ren Ogata,
  • Kotomi Katori,
  • Kazuo Hosokawa,
  • Mizuo Maeda,
  • Akihiko Kikuchi

摘要

Extracellular vesicles (EVs) have emerged as promising biomarkers for liquid biopsies because of their responsiveness to various physiological conditions. However, their dynamic physical properties pose significant analytical challenges, which necessitates the development of novel detection methods. In this study, a portable surface-functionalized power-free microchip (SF-PF microchip) was developed that enables the detection of EVs without external pumps. In particular, the SF-PF microchip specifically detected EVs derived from metastatic breast cancer cells with a limit of detection of 5.0 × 1010 particles/mL. Moreover, the SF-PF microchip required only 2.0 µL of sample volume and completed the detection within approximately 20 min, offering a rapid and convenient approach for EV analysis. Furthermore, the SF-PF microchip successfully detected EVs in serum samples, which highlights its potential for clinical application in liquid biopsies.

Graphical abstract