Squaraine dye as a fluorescent probe for highly sensitive detection of pyrophosphate and alkaline phosphatase

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发表日期：

2024年12月26日

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Wenxuan Zhu,
Shuhua Zhao,
Bei Yu,
Yucun Tao,
Chaoyang Wang,
Lei Shi,
Xiufeng Zhang,
Jing Meng,
Chengqiu Wu,
Chunguang Ding,
Lijia Yu

摘要

We synthesized a squaraine dye (F-0) to develop a method for detecting pyrophosphate (PPi) and alkaline phosphatase (ALP) by modulating the fluorescence of F-0. The fluorescence intensity of the F-0 system was quenched upon the addition of Cu²⁺ ions; however, it was restored when PPi was introduced due to the formation of a complex between PPi and Cu²⁺. Since ALP can hydrolyze PPi, the fluorescence of the system was quenched again upon the addition of ALP. Based on these principles, we established a fluorescent probe that exhibits an "off–on–off" fluorescence response. The detection limits of this method for PPi and ALP were 103 nmol dm⁻³ and 0.18 U dm⁻³, respectively. Moreover, this method demonstrates good selectivity and specificity and can be applied to the detection of PPi in actual samples.

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