Scholarlink

Background/Objective <p>The nature of pressure ulcer is ischemia and hypoxia leading to tissue necrosis, and the role of Shh protein in oxygen–glucose deprivation (OGD) may increase the vascular capacity of endothelial progenitor cells (EPCs), thereby reducing the degree of tissue necrosis after pressure ulcer.</p> Purpose of the Study <p>To explore the effects of ischemia on EPCs.</p> Methods <p>We established the EPCs in vitro ischemia model induced by OGD and detected the expression of Sonic Hedgehog (Shh).</p> Results <p>The results show that the expression level of Shh was reduced obviously in EPCs cultured under the OGD conditions. Invasion, migration, and tube formation abilities were significantly decreased in OGD-EPCs, as well as the ratio between the level of serine/threonine kinase B (AKT) and phosphorylated AKT (p-AKT), the ratio between the level of glycogen synthase kinase-3β (GSK-3β) and phosphorylated GSK-3β (p-GSK-3β), and the expression of mammalian target of rapamycin (mTOR) in OGD-EPCs, while they were recovered remarkably after adding smoothened agonist (SAG), insulin-like growth factor-1 (IGF-1), or IGF-1 + SAG.</p> Conclusion <p>In conclusion, invasion, migration, tube formation abilities, and angiogenic factors were increased in OGD-EPCs by activation of the Shh signaling pathway and the Akt/GSK-3β/mTOR pathway.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Effects of Hypoxia (Oxygen Glucose Deprivation) on Endothelial Progenitor Cell Function and Shh Signaling Pathway in Rats

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年7月31日

查看全文
  • Jianhua Wang,
  • Hongyan Zhan,
  • Mingming Wang,
  • Gang Zhao,
  • Jianhua Sun,
  • Hua Song

摘要

Background/Objective

The nature of pressure ulcer is ischemia and hypoxia leading to tissue necrosis, and the role of Shh protein in oxygen–glucose deprivation (OGD) may increase the vascular capacity of endothelial progenitor cells (EPCs), thereby reducing the degree of tissue necrosis after pressure ulcer.

Purpose of the Study

To explore the effects of ischemia on EPCs.

Methods

We established the EPCs in vitro ischemia model induced by OGD and detected the expression of Sonic Hedgehog (Shh).

Results

The results show that the expression level of Shh was reduced obviously in EPCs cultured under the OGD conditions. Invasion, migration, and tube formation abilities were significantly decreased in OGD-EPCs, as well as the ratio between the level of serine/threonine kinase B (AKT) and phosphorylated AKT (p-AKT), the ratio between the level of glycogen synthase kinase-3β (GSK-3β) and phosphorylated GSK-3β (p-GSK-3β), and the expression of mammalian target of rapamycin (mTOR) in OGD-EPCs, while they were recovered remarkably after adding smoothened agonist (SAG), insulin-like growth factor-1 (IGF-1), or IGF-1 + SAG.

Conclusion

In conclusion, invasion, migration, tube formation abilities, and angiogenic factors were increased in OGD-EPCs by activation of the Shh signaling pathway and the Akt/GSK-3β/mTOR pathway.