<p>Integrating pH-variable natural and synthetic polymers into a singular multifunctional scaffold remains a significant challenge in biomaterials engineering. This study aimed to integrate pH-diverse biomaterials, polyvinyl alcohol (PVA), chitosan (CS), collagen, hyaluronic acid (HA), and serum proteins into a multifunctional scaffold with addition of graphene nanoplatelets and bovine serum albumin (BSA) to improve both structural stability and biological performance for tissue regeneration applications and reduce synthetic polymer dependence. A multifunctional composite was created using lyophilization-assisted integration of PVA, CS, collagen, HA, and serum. Then, the scaffolds were fabricated by electrospinning using 1.8% (v/v) chitosan/acetic acid (ChAC) after adding graphene nanoplatelets and BSA. Electrospinning was conducted at an applied voltage of 29–30 kV and a flow rate of 0.4–0.6 mL/h. The scaffolds were characterized by X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), and contact angle measurements. MCF7 cells were seeded to evaluate cell adhesion using toluidine blue (TB) staining. The optimized scaffold showed the highest viscosity (113.7 Pa&#xa0;s) and shear stress (162.6 Pa), promoting uniform fiber formation (average fiber diameter 104 ± 38 nm). XRD peaks at 22.04°, 20.38°, and 23.74° confirmed improved crystallinity. Wettability improved with the contact angle dropping from 74.8 to 52.2° after 60 s. The MTT assay confirmed low cytotoxicity with cell viability &gt; 90% compared to controls. TB staining showed widespread cell attachment and visible spreading, indicating successful cell–scaffold interactions within 48 h. Light microscopy confirmed robust nuclei and early extracellular matrix deposition on the graphene-BSA-containing scaffold prepared in ChAC. The scaffolds were successfully developed and showed an enhanced biological performance, particularly in terms of supporting cell adhesion.</p> Graphical Abstract <p></p>