<p>A new form and approach for carbon-based material, Carbon Quantum Dots (CQDs), whose size range come under 10&#xa0;nm in diameter &amp; exhibit robust fluorescence, are establishing a novel model for several applications including biomedical and imaging. Because of their tunable optical characteristics, eco-friendly, simple synthesis, great photostability, and biological entity-friendly surface functionalization, CQDs have great potential in cell imaging applications. They are a prospective candidate for advanced nanomaterials research as they possess distinctive optical, physical, and chemical properties. Their favourable properties, simplicity of manufacture, and biocompatibility make doped with heteroatoms or polymer composite materials useful. This CQD variant offers particular advantages in bioimaging, phototherapy, gene treatment, and medication delivery. The review commences with a brief introduction that delineates the top-down and bottom-up strategies for the environmentally sustainable Carbon quantum dots fabrication. Additionally, to increase awareness of the many advantages of CQDs for therapeutic applications, we have reviewed their applications in bioimaging and biomedicine, focusing on current and potential future developments.</p> Graphical Abstract <p></p>