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<p>In this study, colloidal platinum nanoparticles (Pt NPs) were synthesized using the cold plasma technique. The important parameters of this technique for synthesizing platinum nanoparticles depend on the precursor concentration and exposure time to cold plasma. The nanoparticles were examined utilizing UV-Visible spectroscopy and X-ray diffraction (XRD), FESEM, and TEM. The surface plasmon resonance of the synthesized Pt NPs was centered at 202 and 205&#xa0;nm. The XRD pattern showed that the strong, intense peaks indicate a face-centered cubic structure and crystalline nature of the platinum nanoparticles. The crystallite size of the Pt NPs ranged from 25 to 29.37&#xa0;nm. FESEM was used to analyze the morphology of the Pt NPs. According to observations, the synthesized Pt NPs are well dispersed with average particle sizes of 53 and 38&#xa0;nm. Platinum nanoparticles exhibited antibacterial activity against Gram-negative bacteria, with inhibition zones of 20&#xa0;mm and 16.5&#xa0;mm against <i>Pseudomonas aeruginosa</i> and <i>Klebsiella pneumoniae</i>, respectively, at a concentration of 15 ppm (particle size 53&#xa0;nm). At 25 ppm (particle size 38&#xa0;nm), the inhibition zones were 23&#xa0;mm and 18&#xa0;mm, respectively. In contrast, the particles showed no effect against the Gram-positive bacterium <i>Staphylococcus aureus</i>. These results indicate the selective activity of platinum nanoparticles against Gram-negative bacteria. Tests have shown that the effectiveness of platinum nanoparticles depends on their size and concentration.</p>

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Assessment of Platinum Nanoparticles Developed with the Cold Plasma Technique for Antibacterial Activity

  • 期刊论文

  • 发表日期：

    2026年2月17日

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  • Hamsa A. Ali,
  • Zena E. Slaiby,
  • Ruqayah Amer Najm,
  • Jamal M. Rzaij

摘要

In this study, colloidal platinum nanoparticles (Pt NPs) were synthesized using the cold plasma technique. The important parameters of this technique for synthesizing platinum nanoparticles depend on the precursor concentration and exposure time to cold plasma. The nanoparticles were examined utilizing UV-Visible spectroscopy and X-ray diffraction (XRD), FESEM, and TEM. The surface plasmon resonance of the synthesized Pt NPs was centered at 202 and 205 nm. The XRD pattern showed that the strong, intense peaks indicate a face-centered cubic structure and crystalline nature of the platinum nanoparticles. The crystallite size of the Pt NPs ranged from 25 to 29.37 nm. FESEM was used to analyze the morphology of the Pt NPs. According to observations, the synthesized Pt NPs are well dispersed with average particle sizes of 53 and 38 nm. Platinum nanoparticles exhibited antibacterial activity against Gram-negative bacteria, with inhibition zones of 20 mm and 16.5 mm against Pseudomonas aeruginosa and Klebsiella pneumoniae, respectively, at a concentration of 15 ppm (particle size 53 nm). At 25 ppm (particle size 38 nm), the inhibition zones were 23 mm and 18 mm, respectively. In contrast, the particles showed no effect against the Gram-positive bacterium Staphylococcus aureus. These results indicate the selective activity of platinum nanoparticles against Gram-negative bacteria. Tests have shown that the effectiveness of platinum nanoparticles depends on their size and concentration.