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<p>The present study is focused on the synthesis and characterization of zinc oxide nanoparticles (ZnO NPs) using the aqueous extract of <i>Indigofera aspalathoides</i>. The synthesized ZnO NPs were characterized using UV–visible spectroscopy (UV–vis), X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR), Thermogravimetric analysis (TGA), Field emission scanning electron microscopy (FESEM), and Energy-dispersive X-ray spectroscopy (EDX). UV–vis analysis displayed a strong peak at 270&#xa0;nm. The FTIR analysis spectrum exhibited the presence of various functional groups. XRD analysis revealed the crystalline nature with an average size of 107&#xa0;nm. The presence of secondary metabolites, such as flavonoids, anthraquinone, proteins, carbohydrates, and saponins in aqueous extract of <i>I. aspalathoides</i> were attributed to formation of ZnO NPs and which serve as stabilizing and capping agents. FESEM images depicted the spherical shape and EDX analysis peaks confirmed the presence of Zn, and O which confirmed the formation of ZnO NPs. A total weight loss of 48% was observed using thermogravimetric analysis. The 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay was utilized to analyze the antioxidant activity&#xa0;of <i>I. aspalathoides-</i>ZnO NPs and prove 98% of free radical inhibition. The study effectively demonstrates an easy and eco-friendly method for the synthesis of well-organized multifunctional ZnO NPs by green chemistry synthetic approach.</p>

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Bio-synthesis of Zinc Oxide Nanoparticles with Antioxidant Potential Using Indigofera aspalathoides via Green Chemistry Approach

  • 期刊论文

  • 发表日期：

    2025年7月22日

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  • Nivetha Sri Pragatheeswaran,
  • Rajiv Periakaruppan

摘要

The present study is focused on the synthesis and characterization of zinc oxide nanoparticles (ZnO NPs) using the aqueous extract of Indigofera aspalathoides. The synthesized ZnO NPs were characterized using UV–visible spectroscopy (UV–vis), X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR), Thermogravimetric analysis (TGA), Field emission scanning electron microscopy (FESEM), and Energy-dispersive X-ray spectroscopy (EDX). UV–vis analysis displayed a strong peak at 270 nm. The FTIR analysis spectrum exhibited the presence of various functional groups. XRD analysis revealed the crystalline nature with an average size of 107 nm. The presence of secondary metabolites, such as flavonoids, anthraquinone, proteins, carbohydrates, and saponins in aqueous extract of I. aspalathoides were attributed to formation of ZnO NPs and which serve as stabilizing and capping agents. FESEM images depicted the spherical shape and EDX analysis peaks confirmed the presence of Zn, and O which confirmed the formation of ZnO NPs. A total weight loss of 48% was observed using thermogravimetric analysis. The 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay was utilized to analyze the antioxidant activity of I. aspalathoides-ZnO NPs and prove 98% of free radical inhibition. The study effectively demonstrates an easy and eco-friendly method for the synthesis of well-organized multifunctional ZnO NPs by green chemistry synthetic approach.