<p>Cubosomes are nanostructured liquid crystalline particles characterized by their honeycombed internal structure and cubic lattice arrangement. These self-assembled systems, formed by hydrating amphiphilic lipids and stabilized with polymers, offer exceptional stability, biocompatibility, and bio-adhesive properties, making them an advanced platform for versatile drug delivery. Cubosomes can encapsulate a wide range of molecules, including hydrophobic, hydrophilic, and amphiphilic drugs, enabling targeted and controlled release. Their high internal surface area, solid-like rheology, and thermodynamic stability enhance the solubility and bioavailability of poorly water-soluble drugs, ensuring sustained release and efficient drug trapping. Suitable for various administration routes oral, parenteral, mucosal, and transdermal cubosomes offer therapeutic potential for conditions affecting the skin, hair, and systemic tissues. They have demonstrated particular promise in oncology, especially in melanoma therapy, due to their enhanced permeability and retention effects. Beyond drug delivery, cubosomes have been explored for applications as biosensors, artificial cells, and membrane bioreactors. This review explores the fundamental aspects of cubosomes, including their structure, classification, properties, preparation methods, drug loading strategies, and characterization techniques. It compares cubosomes with other nanocarriers, such as liposomes, highlighting their superior stability and resistance to dilution. The review also discusses the challenges associated with cubosome technology, including scalability and manufacturing, while emphasizing their growing role in pharmaceutical, medical, and cosmetic fields. Finally, it explores the potential applications of cubosomes in potential to drive future innovations in drug delivery and biomedicine.</p> Graphical Abstract <p></p>