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<p><i>Ixora coccinea</i>, a perennial shrub belonging to the Rubiaceae family, has been traditionally used to treat various ailments, such as hepatic disorders, cancer, microbial infections, pain, and inflammation. This study investigated the wound-healing potential of iron oxide nanoparticles (IONPs) synthesized from <i>Ixora coccinea</i> flower extract using microwave-assisted methods. The synthesized IONPs were characterized using UV–Visible spectroscopy, FTIR, X-ray diffraction, EDX, and SEM. Phytochemical analysis revealed the presence of phenolic compounds, flavonoids, and other compounds in aqueous extracts. IONPs exhibited significant anti-inflammatory activity in the egg albumin denaturation assay (83% inhibition at 50&#xa0;μg/ml) and membrane stabilization assay (91% inhibition at 50&#xa0;μg/ml). The antioxidant activity of IONPs was evaluated using DPPH, H<sub>2</sub>O<sub>2</sub>, and nitric oxide radical scavenging assays. IONPs showed higher inhibition than ascorbic acid and vitamin C. The antimicrobial activity of IONPs was tested against <i>Pseudomonas</i> sp., <i>Staphylococcus aureus</i>, <i>E. coli</i>, and <i>E. faecalis</i>, with Pseudomonas exhibiting maximum inhibition zones at 50&#xa0;μg/mL and 100&#xa0;μg/ml. The anticoagulant activity of IONPs was assessed, and the highest activity was observed at 50&#xa0;μg/ml with a clot lysis time of 16&#xa0;min. In vitro wound-healing assays revealed the proliferative and migratory effects of IONPs on mouse fibroblasts. The cytotoxicity of the IONPs was evaluated using the MTT assay, which showed no significant toxicity at doses (50–100&#xa0;μg/ml). In conclusion, IONPs synthesized from <i>Ixora coccinea</i> flower extract demonstrated promising anti-inflammatory, antioxidant, antimicrobial, anticoagulant, and wound-healing properties, suggesting their potential for the development of enhanced therapeutic solutions.</p>

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Biosynthesis and Characterization of Ixora coccinea-Based Iron Oxide Nanoparticles: Potential Applications in Wound Healing and Therapeutics

  • 期刊论文

  • 发表日期：

    2025年3月4日

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  • Pavithra Bharathy,
  • Punniyakoti V. Thanikachalam,
  • Silpa Jayaprakash,
  • Prena Dowlath,
  • Praveen Madhaiyan

摘要

Ixora coccinea, a perennial shrub belonging to the Rubiaceae family, has been traditionally used to treat various ailments, such as hepatic disorders, cancer, microbial infections, pain, and inflammation. This study investigated the wound-healing potential of iron oxide nanoparticles (IONPs) synthesized from Ixora coccinea flower extract using microwave-assisted methods. The synthesized IONPs were characterized using UV–Visible spectroscopy, FTIR, X-ray diffraction, EDX, and SEM. Phytochemical analysis revealed the presence of phenolic compounds, flavonoids, and other compounds in aqueous extracts. IONPs exhibited significant anti-inflammatory activity in the egg albumin denaturation assay (83% inhibition at 50 μg/ml) and membrane stabilization assay (91% inhibition at 50 μg/ml). The antioxidant activity of IONPs was evaluated using DPPH, H2O2, and nitric oxide radical scavenging assays. IONPs showed higher inhibition than ascorbic acid and vitamin C. The antimicrobial activity of IONPs was tested against Pseudomonas sp., Staphylococcus aureus, E. coli, and E. faecalis, with Pseudomonas exhibiting maximum inhibition zones at 50 μg/mL and 100 μg/ml. The anticoagulant activity of IONPs was assessed, and the highest activity was observed at 50 μg/ml with a clot lysis time of 16 min. In vitro wound-healing assays revealed the proliferative and migratory effects of IONPs on mouse fibroblasts. The cytotoxicity of the IONPs was evaluated using the MTT assay, which showed no significant toxicity at doses (50–100 μg/ml). In conclusion, IONPs synthesized from Ixora coccinea flower extract demonstrated promising anti-inflammatory, antioxidant, antimicrobial, anticoagulant, and wound-healing properties, suggesting their potential for the development of enhanced therapeutic solutions.