<p>The primary aim of this study is to examine how three-dimensional perception is influenced by different spatial cues in virtual reality (VR) environments. The study investigates various approaches designed to enhance participants’ three-dimensional perception and evaluates the effect of the relationship between spatial cues used in the virtual environment and human scale on three-dimensional perception. Within the framework of an experimental design, scenes featuring different size values and spatial cues were created and presented to the participants. Data obtained from a total of 42 participants were analyzed, and the findings were interpreted. The results indicate that spatial cues aligned with human scale significantly enhance three-dimensional perception in VR. Similarly, participants’ knowledge of three-dimensional structures and spatial proportions also exerts a positive effect on three-dimensional perception. Additionally, the use of VR was observed to improve participants’ concentration levels. Collectively, these findings support the potential of virtual reality environments to provide high validity for experimental studies focused on spatial perception.</p>