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<p>This work investigated the application of mycelium as a potential binder for pavement infrastructure; the focus was on assessing the maturity level of mycelium-bound aggregate specimens. A review of the technical literature revealed that none of the existing methods are transferable to the proposed application. Thus, a new non-destructive laboratory setup was designed and built for this purpose; it is a device for producing shallow depth-sensing indentation. The underlying idea is that the specimen’s indentation stiffness increases as the mycelium matures, and stops evolving once 100% maturity is achieved. Thus, by performing periodic indentation tests during incubation, it becomes possible to observe and quantify the progression of maturity. The work included a demonstration of the setup, with specimens periodically indented over a two-week incubation period. As expected, the average indentation stiffness increased over time. The indentation results were independently validated against CO<InlineEquation ID="IEq1"> <EquationSource Format="TEX">\(_2\)</EquationSource> </InlineEquation> production rate measurements. Overall, it is concluded that shallow depth-sensing indentation is a viable approach for assessing the maturity of mycelium-bound aggregate specimens. Two additional advantages of the approach are: (i) testing can potentially be done outdoors, and (ii) indentation results can produce inputs to mechanistic pavement models.</p>

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Assessing the maturity level of mycelium-bound aggregate specimens through indentation testing

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年3月11日

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  • Yike Yin,
  • Fredrik Johansson,
  • Eyal Levenberg

摘要

This work investigated the application of mycelium as a potential binder for pavement infrastructure; the focus was on assessing the maturity level of mycelium-bound aggregate specimens. A review of the technical literature revealed that none of the existing methods are transferable to the proposed application. Thus, a new non-destructive laboratory setup was designed and built for this purpose; it is a device for producing shallow depth-sensing indentation. The underlying idea is that the specimen’s indentation stiffness increases as the mycelium matures, and stops evolving once 100% maturity is achieved. Thus, by performing periodic indentation tests during incubation, it becomes possible to observe and quantify the progression of maturity. The work included a demonstration of the setup, with specimens periodically indented over a two-week incubation period. As expected, the average indentation stiffness increased over time. The indentation results were independently validated against CO \(_2\) production rate measurements. Overall, it is concluded that shallow depth-sensing indentation is a viable approach for assessing the maturity of mycelium-bound aggregate specimens. Two additional advantages of the approach are: (i) testing can potentially be done outdoors, and (ii) indentation results can produce inputs to mechanistic pavement models.