Scholarlink

<p>Groundwater is an important resource where potable pipe-borne water is lacking. Albeit, yeast-contaminated water is an emerging threat posing a severe risk to public health. This study investigated the occurrence, drug-resistance profile, and virulence potential of yeasts isolated from groundwater sources. Grab groundwater samples were obtained during arid and wet periods, and analysed for yeast enumeration and isolation following standard protocols. The isolates were characterized using phenotypic and molecular approaches and assessed for antibiotic resistance employing standard antifungal agents. Biofilm formation and enzymatic activities including phospholipase, proteinase, lipase, and haemolysin were evaluated. Yeast counts were between 0 and 4 CFU/mL, across both seasons, and 52 yeasts were recovered. The identification and the occurrence of the yeasts were as follows: <i>Candida orthopsilosis</i> (28, 53.8%), <i>Cutaneotrichosporon curvatum</i> (12, 23.1%), <i>Pichia guilliermondii</i> (4, 7.7%), and <i>Pichia kudriavzevii</i> (8, 15.4%). Resistance profile of the isolates was fluconazole (12, 92.30%), flucytosine and amphotericin B (9, 69.23%), and ketoconazole (6, 46.15%). Thirty (30, 58%) isolates were multidrug-resistant, with the highest MARP, 4 (KCA-FLU-AFY-AMB), obtained across the samples. MARI was between 0.75 and 1.00, suggesting the isolates were from sources where antifungals were frequently used. All the isolates were weak biofilm producers, also some showed activity for the hydrolytic enzyme studied. Findings from this study highlight the need for effective monitoring of yeast contamination and antifungal surveillance in groundwater sources. Proper treatment of groundwater is also essential for quality and safe human consumption.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Investigating an emerging menace in groundwater sources: the occurrence, antifungal resistance phenotypes, and virulence potential of yeast contaminants

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年10月14日

查看全文
  • Mobolaji Adenike Titilawo,
  • Oluwagbeminiyi Temitope Esan,
  • Deborah IleriOluwa Oyetunbi,
  • Yinka Titilawo,
  • Janet Olubukola Olaitan

摘要

Groundwater is an important resource where potable pipe-borne water is lacking. Albeit, yeast-contaminated water is an emerging threat posing a severe risk to public health. This study investigated the occurrence, drug-resistance profile, and virulence potential of yeasts isolated from groundwater sources. Grab groundwater samples were obtained during arid and wet periods, and analysed for yeast enumeration and isolation following standard protocols. The isolates were characterized using phenotypic and molecular approaches and assessed for antibiotic resistance employing standard antifungal agents. Biofilm formation and enzymatic activities including phospholipase, proteinase, lipase, and haemolysin were evaluated. Yeast counts were between 0 and 4 CFU/mL, across both seasons, and 52 yeasts were recovered. The identification and the occurrence of the yeasts were as follows: Candida orthopsilosis (28, 53.8%), Cutaneotrichosporon curvatum (12, 23.1%), Pichia guilliermondii (4, 7.7%), and Pichia kudriavzevii (8, 15.4%). Resistance profile of the isolates was fluconazole (12, 92.30%), flucytosine and amphotericin B (9, 69.23%), and ketoconazole (6, 46.15%). Thirty (30, 58%) isolates were multidrug-resistant, with the highest MARP, 4 (KCA-FLU-AFY-AMB), obtained across the samples. MARI was between 0.75 and 1.00, suggesting the isolates were from sources where antifungals were frequently used. All the isolates were weak biofilm producers, also some showed activity for the hydrolytic enzyme studied. Findings from this study highlight the need for effective monitoring of yeast contamination and antifungal surveillance in groundwater sources. Proper treatment of groundwater is also essential for quality and safe human consumption.