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<p>The occurrence of pathogenic <i>Vibrio</i> species in wastewater treatment plant (WWTP) effluents and the effluent-receiving waters is a significant risk to human health and needs to be monitored appropriately. This study aimed to assess the possibility of the Nigerian Abuja WWTP in serving as a reservoir for <i>Vibrio</i> species. Over a period of 12&#xa0;months, thirty-six sewage/water samples were collected from three sampling points in the treatment plant and the Wupa River and analysed using standard microbiological procedures. Preliminary identification studies based on cultural morphology, microscopy, and biochemical tests revealed 24 presumptive <i>Vibrio</i> isolates that were further confirmed by Sanger Sequencing. Among the confirmed isolates, <i>Vibrio cholerae</i> represented the major species (62.5%), followed by <i>V</i>. <i>halioticoli</i> (12.5%), and <i>V. porteresiae</i> (8.33%). Also identified were <i>V. parahaemolyticus, V. comitans, V. furnissii,</i> and <i>V. breoganii,</i> each accounting for 4.17% of the total <i>Vibrio</i> isolates. PCR-based screening of the identified <i>V. cholerae</i> isolates for virulence genes such as the toxin co-regulated pilus gene and cholera toxin-producing gene showed the presence of the former in 80% of the isolates, and the latter in 46.7%. Overall, this study highlights the need to subject effluents from the Abuja WWTP to further sterilization processes before discharge into the nearby river bodies.</p>

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Wastewater treatment plants as reservoirs for Vibrio species: an assessment of the Abuja wastewater treatment plant in Nigeria

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年4月30日

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  • Ini-Abasi Ikinwot Ibangha,
  • Stella Ngozi Madueke,
  • Sylvester Onyebuchi Onuora,
  • Matthew Chekwube Enebe,
  • Paul Isaac Ojodale,
  • Mariana Erasmus,
  • Vincent Nnamdigadi Chigor

摘要

The occurrence of pathogenic Vibrio species in wastewater treatment plant (WWTP) effluents and the effluent-receiving waters is a significant risk to human health and needs to be monitored appropriately. This study aimed to assess the possibility of the Nigerian Abuja WWTP in serving as a reservoir for Vibrio species. Over a period of 12 months, thirty-six sewage/water samples were collected from three sampling points in the treatment plant and the Wupa River and analysed using standard microbiological procedures. Preliminary identification studies based on cultural morphology, microscopy, and biochemical tests revealed 24 presumptive Vibrio isolates that were further confirmed by Sanger Sequencing. Among the confirmed isolates, Vibrio cholerae represented the major species (62.5%), followed by V. halioticoli (12.5%), and V. porteresiae (8.33%). Also identified were V. parahaemolyticus, V. comitans, V. furnissii, and V. breoganii, each accounting for 4.17% of the total Vibrio isolates. PCR-based screening of the identified V. cholerae isolates for virulence genes such as the toxin co-regulated pilus gene and cholera toxin-producing gene showed the presence of the former in 80% of the isolates, and the latter in 46.7%. Overall, this study highlights the need to subject effluents from the Abuja WWTP to further sterilization processes before discharge into the nearby river bodies.