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<p>This study evaluated <i>Candida albicans</i> gene expression and local inflammatory response from mice with oral candidiasis that were treated with DNase I plus antimicrobial photodynamic therapy (aPDT). DNase I was applied, followed by aPDT mediated by Photodithazine®&#xa0;and LED light, singly or combined for five consecutive days. A group was only inoculated with fluconazole-susceptible (CaS) or—resistant (CaR) <i>C. albicans</i> strains. Immediately and 7 days after treatments, <i>C. albicans</i> colonies recovered from mice tongues were used for gene expression evaluation. Tongues from mice killed 24 h and 7 days after treatments were used to evaluate the host’s inflammatory cytokines using cytometric bead array. A reduction occurred in the gene expression related to adhesion to the host substrate, the biofilm extracellular matrix, and oxidative stress of CaS and CaR recovered from mice treated with DNAse I plus aPDT. Increased production of IL-6, TNF-α, and MCP-1 occurred in mice infected with CaS but not as much for those infected with CaR in the groups treated with DNase I plus aPDT and aPDT. DNase I plus aPDT decreased the expression of the fungal genes evaluated and increased local inflammatory response, which helped to fight the infection.</p> Graphical abstract <p></p>

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The association of DNase I with antimicrobial photodynamic therapy affects Candida albicans gene expression and promotes immunomodulatory effects in mice with candidiasis

  • 期刊论文

  • 发表日期：

    2025年5月5日

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  • Cláudia Carolina Jordão,
  • Marlise Inêz Klein,
  • Paula Aboud Barbugli,
  • Túlio Morandin Ferrisse,
  • Juliana Cerini Grassi de Moraes,
  • Ana Claudia Pavarina

摘要

This study evaluated Candida albicans gene expression and local inflammatory response from mice with oral candidiasis that were treated with DNase I plus antimicrobial photodynamic therapy (aPDT). DNase I was applied, followed by aPDT mediated by Photodithazine® and LED light, singly or combined for five consecutive days. A group was only inoculated with fluconazole-susceptible (CaS) or—resistant (CaR) C. albicans strains. Immediately and 7 days after treatments, C. albicans colonies recovered from mice tongues were used for gene expression evaluation. Tongues from mice killed 24 h and 7 days after treatments were used to evaluate the host’s inflammatory cytokines using cytometric bead array. A reduction occurred in the gene expression related to adhesion to the host substrate, the biofilm extracellular matrix, and oxidative stress of CaS and CaR recovered from mice treated with DNAse I plus aPDT. Increased production of IL-6, TNF-α, and MCP-1 occurred in mice infected with CaS but not as much for those infected with CaR in the groups treated with DNase I plus aPDT and aPDT. DNase I plus aPDT decreased the expression of the fungal genes evaluated and increased local inflammatory response, which helped to fight the infection.

Graphical abstract