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<p>Enzyme cost is the one of the constraint in enzymatic hydrolysis of biomass for the production of bioethanol. Utilization of crude enzymes can be a viable alternative to make the bioethanol production process cost-effective, as their preparation requires minimal processing, thus cutting the expensive purification stages. So, to reduce the expense of the bioethanol production, the potential of the isolated strain <i>Aspergillus flavus</i> ITCC 11694.22 was investigated for enzyme production to hydrolyze wheat straw at optimized conditions under submerged fermentation. Several parameters including pH, temperature, inoculum size, incubation time, and carbon and nitrogen concentrations have been optimized utilizing a one variable at a time method and Response Surface Methodology (RSM). The optimum pH and temperature found for cellulase and xylanase activity were 6 and 35&#xa0;°C. The highest CMCase, FPase and xylanase activity achieved were 29.7 U/g, 17.41 U/g and 403.84 U/g, respectively at optimum conditions of 84&#xa0;h incubation time, 5% inoculum size, 1% wheat straw and 0.35% nitrogen concentration. Commercial (diluted) and crude enzyme yields reducing sugars of 6.92&#xa0;mg/ml and 4.78&#xa0;mg/ml, respectively which were further fermented to produce ethanol. Maximum ethanol efficiency achieved was 86.5% from <i>Saccharomyces cerevisiae</i> utilizing commercial enzyme treated hydrolyzate.</p>

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Wheat Straw Valorization for Ethanol Production using Isolated Aspergillus flavus ITCC 11694.22

  • 期刊论文

  • 发表日期：

    2026年5月19日

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  • Arti Devi,
  • Anita Singh,
  • Richa Kothari

摘要

Enzyme cost is the one of the constraint in enzymatic hydrolysis of biomass for the production of bioethanol. Utilization of crude enzymes can be a viable alternative to make the bioethanol production process cost-effective, as their preparation requires minimal processing, thus cutting the expensive purification stages. So, to reduce the expense of the bioethanol production, the potential of the isolated strain Aspergillus flavus ITCC 11694.22 was investigated for enzyme production to hydrolyze wheat straw at optimized conditions under submerged fermentation. Several parameters including pH, temperature, inoculum size, incubation time, and carbon and nitrogen concentrations have been optimized utilizing a one variable at a time method and Response Surface Methodology (RSM). The optimum pH and temperature found for cellulase and xylanase activity were 6 and 35 °C. The highest CMCase, FPase and xylanase activity achieved were 29.7 U/g, 17.41 U/g and 403.84 U/g, respectively at optimum conditions of 84 h incubation time, 5% inoculum size, 1% wheat straw and 0.35% nitrogen concentration. Commercial (diluted) and crude enzyme yields reducing sugars of 6.92 mg/ml and 4.78 mg/ml, respectively which were further fermented to produce ethanol. Maximum ethanol efficiency achieved was 86.5% from Saccharomyces cerevisiae utilizing commercial enzyme treated hydrolyzate.