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<p>This article describes the isolation and characterization of a very efficient cellulolytic bacterial isolate, CS1, with dual application in lignocellulosic biomass breakdown and plant growth promotion. CS1 was identified as <i>Escherichia coli</i> by 16SrRNA gene sequencing (GenBank accession no. PV731524) and is a Gram-negative, catalase-positive bacterium which gave evident hydrolysis zones on Congo red-stained cellulose agar, reflecting strong cellulase activity. The strain had a cellulolytic index of 2.1 and maximum CMCase activity of 35&#xa0;°C and 3% carboxymethyl cellulose (CMC), as assessed by the DNS method. CS1 was proved to be safe to use in the environment, with a low multiple antibiotic resistance (MAR) index of 0.200 and no hemolytic activity. Maximum growth of CS1 were maximized through Response Surface Methodology (RSM), which provided a statistically significant model (F = 15.94, <i>p</i> &lt; 0.05) and revealed optimal activity conditions of 30&#xa0;°C, 3%CMC, and 2–3% inoculum. The CS1 strain exhibited efficient food waste and rice straw degradation with increased enzyme activity (2.03U/mL) and structural breakdown viewed under light microscopy. FTIR analysis showed dramatic decreases in cellulose, hemicellulose, and lignin peaks. These outcomes identify CS1’s excellent prospects for bioconversion of agricultural waste into biofuel. With such optimized conditions, CS1 efficiently broke down rice straw, promoting carbon loss and decomposition kinetics. Pot experiments with <i>Vigna radiata</i> showed significant increases in root and shoot biomass as well as sugar content, even with raw rice straw. These findings put CS1 in the position of a useful bioinoculant for sustainable agriculture, combining waste valorization with crop productivity improvement.</p> Graphical Abstract <p></p>

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Harnessing Isolated Bacteria for Lignocellulose Degradation and Plant Growth Promotion: Experimental and In Silico Strategies Towards Sustainable Agriculture and a Circular Bioeconomy

  • 期刊论文

  • 发表日期：

    2026年4月8日

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  • Bidisha Das,
  • Soumita Maji,
  • Debalina Samanta,
  • Arvin Ahmed,
  • Suryadeep Kanji,
  • Ujjal Dey,
  • Maman Sidhanta,
  • Gargi Roy Chowdhury,
  • Sandhimita Mondal

摘要

This article describes the isolation and characterization of a very efficient cellulolytic bacterial isolate, CS1, with dual application in lignocellulosic biomass breakdown and plant growth promotion. CS1 was identified as Escherichia coli by 16SrRNA gene sequencing (GenBank accession no. PV731524) and is a Gram-negative, catalase-positive bacterium which gave evident hydrolysis zones on Congo red-stained cellulose agar, reflecting strong cellulase activity. The strain had a cellulolytic index of 2.1 and maximum CMCase activity of 35 °C and 3% carboxymethyl cellulose (CMC), as assessed by the DNS method. CS1 was proved to be safe to use in the environment, with a low multiple antibiotic resistance (MAR) index of 0.200 and no hemolytic activity. Maximum growth of CS1 were maximized through Response Surface Methodology (RSM), which provided a statistically significant model (F = 15.94, p < 0.05) and revealed optimal activity conditions of 30 °C, 3%CMC, and 2–3% inoculum. The CS1 strain exhibited efficient food waste and rice straw degradation with increased enzyme activity (2.03U/mL) and structural breakdown viewed under light microscopy. FTIR analysis showed dramatic decreases in cellulose, hemicellulose, and lignin peaks. These outcomes identify CS1’s excellent prospects for bioconversion of agricultural waste into biofuel. With such optimized conditions, CS1 efficiently broke down rice straw, promoting carbon loss and decomposition kinetics. Pot experiments with Vigna radiata showed significant increases in root and shoot biomass as well as sugar content, even with raw rice straw. These findings put CS1 in the position of a useful bioinoculant for sustainable agriculture, combining waste valorization with crop productivity improvement.

Graphical Abstract