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<p>The transcriptional regulation of trehalose metabolism in <i>Helicoverpa armigera</i>, a major lepidopteran pest, remains largely unexplored despite the established importance of trehalose in insect physiology. This study investigates the regulatory role of transcription factors (TF) in modulating trehalose metabolism. Promotor analysis (prediction of TF binding site) and developmental stage expression analysis suggested that two TFs, <i>Haftz</i> and <i>Hadfd</i>, may play significant role in regulating trehalose metabolism. RNAi-mediated silencing of <i>Haftz</i> and <i>Hadfd</i> did not affect larval phenotype or body weight; however, significant transcriptional and metabolic alterations were observed. <i>Haftz</i> silencing downregulated <i>HaTreh</i> and <i>HaST64</i>, leading to increased trehalose, T6P, G6P levels. While <i>Hadfd</i> silencing modestly upregulated <i>HaTPS</i> and <i>HaTreh-1</i> expression and reduced G6P and T6P levels. Importantly, <i>Ha</i>DFD was confirmed to directly bind <i>HaTPS</i> and <i>HaTreh-1</i> promoters via microscale thermophoresis and <i>in silico</i> docking, establishing it as a direct transcriptional regulator. This study provides mechanistic insights into the upstream regulation of trehalose metabolism and identifies <i>Hadfd</i> as a promising molecular target. These findings open new avenues for precise metabolic disruption in pest management strategies.</p>

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Embryonic transcription factors act as regulators of trehalose metabolism in Helicoverpa armigera

  • 期刊论文

  • 发表日期：

    2026年1月28日

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  • Meenakshi B. Tellis,
  • Vikram J. Nichit,
  • Rakesh S. Joshi

摘要

The transcriptional regulation of trehalose metabolism in Helicoverpa armigera, a major lepidopteran pest, remains largely unexplored despite the established importance of trehalose in insect physiology. This study investigates the regulatory role of transcription factors (TF) in modulating trehalose metabolism. Promotor analysis (prediction of TF binding site) and developmental stage expression analysis suggested that two TFs, Haftz and Hadfd, may play significant role in regulating trehalose metabolism. RNAi-mediated silencing of Haftz and Hadfd did not affect larval phenotype or body weight; however, significant transcriptional and metabolic alterations were observed. Haftz silencing downregulated HaTreh and HaST64, leading to increased trehalose, T6P, G6P levels. While Hadfd silencing modestly upregulated HaTPS and HaTreh-1 expression and reduced G6P and T6P levels. Importantly, HaDFD was confirmed to directly bind HaTPS and HaTreh-1 promoters via microscale thermophoresis and in silico docking, establishing it as a direct transcriptional regulator. This study provides mechanistic insights into the upstream regulation of trehalose metabolism and identifies Hadfd as a promising molecular target. These findings open new avenues for precise metabolic disruption in pest management strategies.