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<p>Anti-Mullerian hormone (AMH) is a biomarker of reproductive lifespan in cows. In our previous study, we developed an in-house ELISA for bovine AMH. Afterwards, we targeted to validate this ELISA in field condition and to compare the efficacy of this ELISA with other commercial assays. Also, we aimed to standardize the age-specific AMH level in the <i>Bos indicus</i> cows using both assays. For this, blood samples were collected from cows of 0–10 yrs of age (Sahiwal and Tharparkar breed). The minimum AMH level was found at 0–6 months of age (199.07 ± 44.89 and 180.87 ± 32.76 pg/mL for each breeds. Afterwards, it increased significantly (<i>p</i> &lt; 0.05) and reached a peak value between 1.5–2.0 and 2.0–2.5 years in Sahiwal and Tharparkar (1497.71 ± 77.18 &amp; 1725.14 ± 114.81 pg/mL). Afterwards, it starts decreasing with age. Both the assays showed high correlation. The age-specific profiling of AMH was done for the first time in indigenous cows and the in-house ELISA was found as efficient as the commercial assay.</p> Graphical abstract <p></p>

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Field validation of an in-house epitope-specific ELISA for age-specific profiling of anti-müllerian hormone in indigenous cows (Bos indicus)

  • 期刊论文

  • 发表日期：

    2025年11月18日

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  • Prasanna Pal,
  • Anjali Aggarwal,
  • Fanny Josan,
  • Sonika Grewal,
  • Rajib Deb,
  • Sachinandan De

摘要

Anti-Mullerian hormone (AMH) is a biomarker of reproductive lifespan in cows. In our previous study, we developed an in-house ELISA for bovine AMH. Afterwards, we targeted to validate this ELISA in field condition and to compare the efficacy of this ELISA with other commercial assays. Also, we aimed to standardize the age-specific AMH level in the Bos indicus cows using both assays. For this, blood samples were collected from cows of 0–10 yrs of age (Sahiwal and Tharparkar breed). The minimum AMH level was found at 0–6 months of age (199.07 ± 44.89 and 180.87 ± 32.76 pg/mL for each breeds. Afterwards, it increased significantly (p < 0.05) and reached a peak value between 1.5–2.0 and 2.0–2.5 years in Sahiwal and Tharparkar (1497.71 ± 77.18 & 1725.14 ± 114.81 pg/mL). Afterwards, it starts decreasing with age. Both the assays showed high correlation. The age-specific profiling of AMH was done for the first time in indigenous cows and the in-house ELISA was found as efficient as the commercial assay.

Graphical abstract