<p><i>Dendrobium nobile</i> is an epiphytic orchid with immense ornamental and medicinal value. However, the molecular mechanisms underlying the growth and development of <i>Dendrobium nobile</i> remain poorly understood<i>.</i> The identification of development-associated molecules is crucial, as they play a significant role in defining plant architecture and in the development of new varieties. This study identifies tissue-specific regulatory pathways mediated by coding and non-coding RNAs during leaf and stem development in <i>Dendrobium nobile</i>. Our results identified differentially expressed mRNAs, long non-coding RNAs (lncRNAs), transcription factors, and key biological processes and pathways associated with leaf and stem development in <i>Dendrobium nobile</i>. In addition, tissue-specific PPI network in leaves identified SAC1, SAC4-2, KAI0495686.1, and SAC2-2 as important hubs engaged in the inositol phosphate metabolism during leaf growth. Furthermore, tissue-specific PPI network in stems identified hubs, viz. CYP704C1-3, KAI0513567.1, CYP77A3-3, and CYP77A1-2 as associated with starch, maltose, and sucrose metabolism. Leaf-specific mRNAs were primarily involved in biosynthetic pathways related to chlorophyll generation for photosynthesis. In contrast, stem-specific mRNAs were involved in pathways related to plant cell wall structural integrity as well as phenolic compound, alkaloid, and polysaccharide production. Developmental regulators belonging to the ARF, GRF, LBD, NAC, and YABBY transcription factor families were revealed to be involved during different stages of leaf and stem development in <i>Dendrobium nobile</i></p>