Study of prostate specific antibody-antigen binding on gold functionalized MoS₂ nanospheres

A vital method for diagnosing diseases, particularly in the detection of cancer, is antibody-antigen binding, prostate cancer is the second most common cancer among men. Prostate cancer is typically diagnosed through prostate specific antigen testing. Here, we synthesized a nanoplatform with gold functionalized molybdenum disulphide (MoS₂) nanospheres, which can be useful for modification of the working electrode of electrochemical sensor for prostate cancer detection. It is based on the identification of prostate specific antigen biomarkers. For functionalization of MoS₂ nanospheres with gold (Au) particles, PVP was employed to ensure that the gold particles were distributed evenly. Prostate-specific monoclonal antibodies were then bound to the surface. Through UV–Vis spectroscopy, its binding reaction and the digesting time impact on antigen- antibody binding were investigated. UV–Vis spectroscopy, FTIR, and FESEM were used to examine the surface morphologies of MoS₂ and Au/MoS₂. The formation of MoS₂ nanospheres with leaves-like structures of 10-12 nm in width was confirmed by FESEM images An additional peak arises in the UV–Vis spectroscopy after the addition of antigen–antibody on Au/MoS₂, confirming the binding and formation of Ag/Ab/AU/MoS₂, i.e., the antigen–antibody-Au/MoS₂ without any use of linker component.