Scholarlink

<p>This study aimed to investigate the chemical constituents of <i>Pseudotsuga menziesii</i> (Mirb.) Franco, Pinaceae, and evaluate their inhibitory activities on nitric oxide production and α-glucosidase activity. The methanolic extract was fractionated, and the ethyl acetate–soluble fraction was subjected to chromatographic separation, yielding seven metabolites, including flavonoids and other phenolic constituents. Among the isolated compounds, apigenin exhibited the strongest inhibition of nitric oxide production (IC<sub>50</sub> 18.1&#xa0;μM), while (+)-taxifolin and (−)-taxifolin showed notable dual activity against NO production (IC<sub>50</sub> 25.2 and 28.6&#xa0;μM, respectively) and α-glucosidase (IC<sub>50</sub> 25.4 and 44.8&#xa0;μM, respectively). Other compounds displayed weak or negligible activity. To provide molecular-level insights, docking analyses were performed against inducible nitric oxide synthase, cyclooxygenase-2, interleukin-8 (IL-8), and <i>Saccharomyces cerevisiae</i> α-glucosidase. Apigenin and (+)-taxifolin showed favorable predicted binding affinities for all evaluated targets, supporting the observed activity trend. Together, these findings identify apigenin and taxifolin derivatives as relevant bioactive constituents of <i>P. menziesii</i> and provide preliminary <i>in vitro</i> and supportive <i>in silico</i> evidence for dual inhibitory potential against nitric oxide production and α-glucosidase.</p> Graphical Abstract <p></p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Inhibitors of Nitric Oxide Production and α-Glucosidase from Pseudotsuga menziesii: An Integrated In Vitro and In Silico Study

  • 期刊论文

  • 发表日期：

    2026年5月29日

查看全文
  • Dao Cuong To,
  • Le Minh Hoang,
  • Phi Hung Nguyen,
  • Thuy Mi Pham Lam,
  • Hoa Thi Nguyen,
  • Thi Viet Hoa Truong,
  • Minh Quan Pham,
  • Thi Thuy Do,
  • Phuong Dai Nguyen Nguyen,
  • Truong Nhan Ngu,
  • Viet An Thi Nguyen,
  • Viet Tuan Trinh

摘要

This study aimed to investigate the chemical constituents of Pseudotsuga menziesii (Mirb.) Franco, Pinaceae, and evaluate their inhibitory activities on nitric oxide production and α-glucosidase activity. The methanolic extract was fractionated, and the ethyl acetate–soluble fraction was subjected to chromatographic separation, yielding seven metabolites, including flavonoids and other phenolic constituents. Among the isolated compounds, apigenin exhibited the strongest inhibition of nitric oxide production (IC50 18.1 μM), while (+)-taxifolin and (−)-taxifolin showed notable dual activity against NO production (IC50 25.2 and 28.6 μM, respectively) and α-glucosidase (IC50 25.4 and 44.8 μM, respectively). Other compounds displayed weak or negligible activity. To provide molecular-level insights, docking analyses were performed against inducible nitric oxide synthase, cyclooxygenase-2, interleukin-8 (IL-8), and Saccharomyces cerevisiae α-glucosidase. Apigenin and (+)-taxifolin showed favorable predicted binding affinities for all evaluated targets, supporting the observed activity trend. Together, these findings identify apigenin and taxifolin derivatives as relevant bioactive constituents of P. menziesii and provide preliminary in vitro and supportive in silico evidence for dual inhibitory potential against nitric oxide production and α-glucosidase.

Graphical Abstract