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<p>A hydroethanolic extract and its nonpolar dichloromethane fraction obtained from roots of <i>Fridericia platyphylla</i> (Cham.) L.G.Lohmann, Bignoniaceae, were evaluated against yeasts (<i>Candida albicans</i>, <i>C. krusei</i>, and <i>Cryptococcus neoformans</i>), and Gram-negative (<i>Escherichia coli</i>) and Gram-positive (<i>Staphylococcus aureus</i>) bacteria. Neither extract showed relevant activity against yeasts or Gram-negative bacteria. In contrast, the dichloromethane fraction exhibited stronger activity against methicillin-sensitive <i>S. aureus</i> (MSSA, ATCC 29213) with minimum inhibitory concentration of 26.7&#xa0;µg/ml and showed marked antioxidant capacity. Three dimeric flavonoids—brachydins A, B, and C—isolated from dichloromethane fraction displayed potent antimicrobial activity against MSSA ATCC 29213 and V459 strains, with brachydin C being the most active (MIC 12.5 and 75&#xa0;µM, respectively). Brachydin C also decreased the activity of <i>S. aureus</i> virulence-associated enzymes (DNase and catalase). Extracts and brachydins inhibited nitric oxide (NO) production by LPS-stimulated RAW264.7 macrophages. The <i>in silico</i> ADME profile was further investigated. In conclusion, the results highlight the potential of brachydin C as an anti-staphylococcal agent and the antibacterial, anti-inflammatory and antioxidant potential of <i>F. platyphylla.</i></p> Graphical Abstract <p></p>

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Antimicrobial and Anti-inflammatory Activities of Brachydins A-C and Root Extracts from Fridericia platyphylla

  • 期刊论文

  • 发表日期：

    2025年12月16日

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  • Inês Lopes,
  • Carla Campos,
  • Fernando Silva,
  • Rui M. Gil da Costa,
  • Layana S. Ferreira,
  • Cláudia Q. da Rocha,
  • Haissa O. Brito,
  • João Paulo Capela,
  • Eugénia Pinto,
  • Rui Medeiros,
  • Fátima Cerqueira

摘要

A hydroethanolic extract and its nonpolar dichloromethane fraction obtained from roots of Fridericia platyphylla (Cham.) L.G.Lohmann, Bignoniaceae, were evaluated against yeasts (Candida albicans, C. krusei, and Cryptococcus neoformans), and Gram-negative (Escherichia coli) and Gram-positive (Staphylococcus aureus) bacteria. Neither extract showed relevant activity against yeasts or Gram-negative bacteria. In contrast, the dichloromethane fraction exhibited stronger activity against methicillin-sensitive S. aureus (MSSA, ATCC 29213) with minimum inhibitory concentration of 26.7 µg/ml and showed marked antioxidant capacity. Three dimeric flavonoids—brachydins A, B, and C—isolated from dichloromethane fraction displayed potent antimicrobial activity against MSSA ATCC 29213 and V459 strains, with brachydin C being the most active (MIC 12.5 and 75 µM, respectively). Brachydin C also decreased the activity of S. aureus virulence-associated enzymes (DNase and catalase). Extracts and brachydins inhibited nitric oxide (NO) production by LPS-stimulated RAW264.7 macrophages. The in silico ADME profile was further investigated. In conclusion, the results highlight the potential of brachydin C as an anti-staphylococcal agent and the antibacterial, anti-inflammatory and antioxidant potential of F. platyphylla.

Graphical Abstract