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<p>Convulsions are known to induce oxidative stress in the brain. <i>Cannabis sativa</i> L., Cannabaceae, is used as an anticonvulsant in refractory epilepsy. Synergistic interactions between plants may enhance therapeutic efficacy and reduce adverse effects. This study aimed to evaluate the antioxidant and anticonvulsant activities of an ethanolic extract of <i>C. sativa</i>, in association with an aqueous extract of <i>Tilia</i> × <i>viridis</i> (Bayer) Simonk., Malvaceae, to assess potential synergistic effects. Therefore, the scavenging effect on diphenyl-2-picrylhydrazyl, inhibition of egg yolk lipid peroxidation, superoxide dismutase and peroxidase-like activities were determined. The anticonvulsant effect was evaluated using a pentylenetetrazole-induced seizure model in zebrafish. HPLC–MS/MS was used to identify flavonoids in <i>C. sativa</i> extract, and HPLC–UV was employed to quantify cannabidiol in <i>C. sativa</i> extract and epicatechin in <i>T.</i> × <i>viridis</i>extract. The antioxidant activities of <i>C. sativa</i> were enhanced when combined with <i>T.</i> × <i>viridis</i>, as demonstrated by improved diphenyl-2-picrylhydrazyl radical scavenging, reduced egg yolk lipid peroxidation, and increased superoxide dismutase activity.&#xa0;The anticonvulsant activity of <i>C. sativa</i> was also potentiated by <i>T.</i> × <i>viridis</i>. This study demonstrates that the combination of <i>C. sativa</i> with <i>T.</i> × <i>viridis</i> significantly enhances both the <i>in vitro</i> antioxidant activity and the <i>in vivo</i> anticonvulsant effect of <i>C. sativa</i> alone.</p> Graphical Abstract <p></p>

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In Vitro Antioxidant Effect and In Vivo Anticonvulsant Activity of the Association Between Cannabis sativa and Tilia × viridis

  • 期刊论文

  • 发表日期：

    2025年4月8日

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  • Elina Malén Saint Martin,
  • Sebastián Graziati,
  • Cecilia Dobrecky,
  • Sabrina Flor,
  • Carla Marrassini,
  • Ignacio Peralta,
  • Laura Cogoi,
  • Gabriela Coux,
  • María Rosario Alonso,
  • Claudia Anesini

摘要

Convulsions are known to induce oxidative stress in the brain. Cannabis sativa L., Cannabaceae, is used as an anticonvulsant in refractory epilepsy. Synergistic interactions between plants may enhance therapeutic efficacy and reduce adverse effects. This study aimed to evaluate the antioxidant and anticonvulsant activities of an ethanolic extract of C. sativa, in association with an aqueous extract of Tilia × viridis (Bayer) Simonk., Malvaceae, to assess potential synergistic effects. Therefore, the scavenging effect on diphenyl-2-picrylhydrazyl, inhibition of egg yolk lipid peroxidation, superoxide dismutase and peroxidase-like activities were determined. The anticonvulsant effect was evaluated using a pentylenetetrazole-induced seizure model in zebrafish. HPLC–MS/MS was used to identify flavonoids in C. sativa extract, and HPLC–UV was employed to quantify cannabidiol in C. sativa extract and epicatechin in T. × viridisextract. The antioxidant activities of C. sativa were enhanced when combined with T. × viridis, as demonstrated by improved diphenyl-2-picrylhydrazyl radical scavenging, reduced egg yolk lipid peroxidation, and increased superoxide dismutase activity. The anticonvulsant activity of C. sativa was also potentiated by T. × viridis. This study demonstrates that the combination of C. sativa with T. × viridis significantly enhances both the in vitro antioxidant activity and the in vivo anticonvulsant effect of C. sativa alone.

Graphical Abstract