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<p>The search for new forms of control against fasciolosis was the aim of the present study, based on the characterization and evaluation of the action of the volatile oil of <i>Origanum vulgare</i> L., Lamiaceae, in its free form or encapsulated in β-cyclodextrin (<i>O. vulgare</i>/β-CD) on the miracidial hatching of <i>Fasciola hepatica</i> eggs. Volatile oil of <i>O. vulgare</i> was characterized by GC-FID and GC–MS, and the major chemical component found was carvacrol (70.31%). <i>Origanum vulgare</i>/β-CD was prepared by the kneading method in a molar ratio of 1:1. <i>Origanum vulgare</i>/β-CD was characterized by FTIR-ATR, PXRD, and thermal analyses, which confirmed signs of interaction between volatile oil of <i>O. vulgare</i> and β-CD, through shifts and intensity changes of absorption peaks, and enhanced thermal stability. <i>Fasciola hepatica</i> eggs exposed to volatile oil of <i>O. vulgare</i> or <i>O. vulgare</i> /β-CD showed 100% inhibition of hatchability from 60 and 40&#xa0;ppm, respectively. The main morphological alteration observed in the eggs after exposure to volatile oil of <i>O. vulgare</i>, <i>O. vulgare</i>/β-CD, or β-CD was the absence of cell division, demonstrating no miracidial development. The interactions between volatile oil of <i>O. vulgare</i> and β-CD resulted in a delivery system capable of enhancing efficacy over time against the trematode compared to both components in their free form. In silico studies involving the interactions between carvacrol and β-tubulin demonstrated a comparable energetic profile and stability within the protein active site between carvacrol and albendazole (positive control). Therefore, this study contributes to comprehending the potential of <i>O. vulgare</i> /β-CD as an innovative delivery system that could serve as a first step toward new therapeutic possibilities in fasciolosis control.</p> Graphical Abstract <p></p>

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Inclusion Complex of Origanum vulgare Volatile Oil with β-Cyclodextrin Enhances Its Fasciolicidal Efficacy

  • 期刊论文

  • 发表日期：

    2025年2月12日

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  • Lais Sperandio Cassani,
  • Ygor Henrique da Silva,
  • Natânia do Carmo Sperandio,
  • Leonardo Oliveira Trivilin,
  • Mariana Belizario de Oliveira,
  • Walter Cesar Celeri Bigui,
  • Arlan da Silva Gonçalves,
  • Adilson Vidal Costa,
  • Vagner Tebaldi de Queiroz,
  • Isabella Vilhena Freire Martins

摘要

The search for new forms of control against fasciolosis was the aim of the present study, based on the characterization and evaluation of the action of the volatile oil of Origanum vulgare L., Lamiaceae, in its free form or encapsulated in β-cyclodextrin (O. vulgare/β-CD) on the miracidial hatching of Fasciola hepatica eggs. Volatile oil of O. vulgare was characterized by GC-FID and GC–MS, and the major chemical component found was carvacrol (70.31%). Origanum vulgare/β-CD was prepared by the kneading method in a molar ratio of 1:1. Origanum vulgare/β-CD was characterized by FTIR-ATR, PXRD, and thermal analyses, which confirmed signs of interaction between volatile oil of O. vulgare and β-CD, through shifts and intensity changes of absorption peaks, and enhanced thermal stability. Fasciola hepatica eggs exposed to volatile oil of O. vulgare or O. vulgare /β-CD showed 100% inhibition of hatchability from 60 and 40 ppm, respectively. The main morphological alteration observed in the eggs after exposure to volatile oil of O. vulgare, O. vulgare/β-CD, or β-CD was the absence of cell division, demonstrating no miracidial development. The interactions between volatile oil of O. vulgare and β-CD resulted in a delivery system capable of enhancing efficacy over time against the trematode compared to both components in their free form. In silico studies involving the interactions between carvacrol and β-tubulin demonstrated a comparable energetic profile and stability within the protein active site between carvacrol and albendazole (positive control). Therefore, this study contributes to comprehending the potential of O. vulgare /β-CD as an innovative delivery system that could serve as a first step toward new therapeutic possibilities in fasciolosis control.

Graphical Abstract