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<p>Cadaverine, a key precursor for nylon, is conventionally produced using free whole-cell lysine decarboxylase systems, with poor recoverability and limited reusability undermining industrial viability. Here, CadA-overexpressing <i>E. coli</i> were entrapped in κ-carrageenan and subsequently crosslinked with polyethylenimine (PEI) to enhance stability and operational longevity. Systematic optimization of gel parameters and CTAB permeabilization enabled robust 100-mL packed-bed reactor directly fed with lysine fermentation broth. The reactor maintained stable operation for 200&#xa0;h, yielding 6.40&#xa0;L of broth containing 112&#xa0;g/L cadaverine at 97.4% conversion and productivity of 44.8&#xa0;g/g<sub>DCW</sub>/h. Immobilized cells reached a total catalyst productivity of 8.96&#xa0;kg cadaverine per gram dry cell weight and 19-fold increase over free cells. These advantages position κ-carrageenan/PEI immobilization as a practical and economical strategy for continuous cadaverine production.</p>

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Continuous cadaverine production using κ-carrageenan/polyethylenimine-immobilized cells with exceptional catalytic performance

  • 期刊论文

  • 发表日期：

    2026年3月17日

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  • Gongcheng Peng,
  • Binhao Wang,
  • Jiayu Cheng,
  • Xiaoyu Wang,
  • Junpeng Sheng,
  • Jieyu Zhou,
  • Rui-Zhi Han,
  • Ye Ni,
  • Jin-Jun Dong

摘要

Cadaverine, a key precursor for nylon, is conventionally produced using free whole-cell lysine decarboxylase systems, with poor recoverability and limited reusability undermining industrial viability. Here, CadA-overexpressing E. coli were entrapped in κ-carrageenan and subsequently crosslinked with polyethylenimine (PEI) to enhance stability and operational longevity. Systematic optimization of gel parameters and CTAB permeabilization enabled robust 100-mL packed-bed reactor directly fed with lysine fermentation broth. The reactor maintained stable operation for 200 h, yielding 6.40 L of broth containing 112 g/L cadaverine at 97.4% conversion and productivity of 44.8 g/gDCW/h. Immobilized cells reached a total catalyst productivity of 8.96 kg cadaverine per gram dry cell weight and 19-fold increase over free cells. These advantages position κ-carrageenan/PEI immobilization as a practical and economical strategy for continuous cadaverine production.