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<p>Cyanide, a toxic compound from plant metabolism, might be accumulated in the plant-based food fermentation. It is important to degrade cyanide in these food fermentations. This study identified a potential cyanide-degradation enzyme gene (<i>nitC</i>) in <i>Bacillus amyloliquefaciens</i> CCTCC M 20242168 with cyanide-degrading activity in Baijiu fermentation. The <i>nitC</i> gene was expressed in <i>Escherichia coli</i> BL21 (DE3), and the purified enzyme successfully degraded cyanide into ammonia and formic acid. The purified nitrilase showed the highest activity (23.7&#xa0;U/g) at the optimal pH of 6.5 and temperature of 35&#xa0;°C. Substrate specificity analysis revealed high catalytic activity toward both cyanide and benzonitrile. Nitrilase NitC also exhibited high enzymatic activity under acidic conditions and its observed tolerance towards 1%–10% ethanol, suggesting its potential application in cyanide bioremediation, particularly under acidic conditions.</p>

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Identification and characterization of nitrilase NitC from Baijiu fermentation and evaluation in cyanide degradation

  • 期刊论文

  • 发表日期：

    2026年1月2日

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  • Yushan Jiang,
  • Zhihao Yao,
  • Qun Wu,
  • Yao Nie

摘要

Cyanide, a toxic compound from plant metabolism, might be accumulated in the plant-based food fermentation. It is important to degrade cyanide in these food fermentations. This study identified a potential cyanide-degradation enzyme gene (nitC) in Bacillus amyloliquefaciens CCTCC M 20242168 with cyanide-degrading activity in Baijiu fermentation. The nitC gene was expressed in Escherichia coli BL21 (DE3), and the purified enzyme successfully degraded cyanide into ammonia and formic acid. The purified nitrilase showed the highest activity (23.7 U/g) at the optimal pH of 6.5 and temperature of 35 °C. Substrate specificity analysis revealed high catalytic activity toward both cyanide and benzonitrile. Nitrilase NitC also exhibited high enzymatic activity under acidic conditions and its observed tolerance towards 1%–10% ethanol, suggesting its potential application in cyanide bioremediation, particularly under acidic conditions.