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<p>1,3-Propanediol is a key monomer for producing polytrimethylene terephthalate. However, most reported microbial production systems rely on costly pure glycerol substrates or require expensive vitamin B12 supplementation, which considerably restricts their economic scalability. To overcome these constraints, a previously isolated <i>Klebsiella pneumoniae</i> strain was engineered to convert crude glycerol to 1,3-propanediol efficiently via adaptive laboratory evolution and rational metabolic engineering. In addition, an adenosine-driven ATP regeneration system was introduced; the engineered strain <i>K. pneumoniae</i> CG6.6 was capable of producing 110&#xa0;g/L 1,3-propanediol in 48&#xa0;h in a 5-L bioreactor, achieving a yield of 0.48&#xa0;g/g without vitamin B12 supplementation. These findings highlight the potential of the engineered <i>K. pneumoniae</i> for sustainable and cost-effective 1,3-propanediol biosynthesis from crude glycerol.</p>

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Metabolic engineering of Klebsiella pneumoniae for 1,3-propanediol production via crude glycerol tolerance enhancement and ATP regeneration

  • 期刊论文

  • 发表日期：

    2025年12月15日

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  • Cong Gao,
  • Shaolun Zhang,
  • Guangjie Liang,
  • Kaifang Liu,
  • Jia Liu,
  • Liming Liu

摘要

1,3-Propanediol is a key monomer for producing polytrimethylene terephthalate. However, most reported microbial production systems rely on costly pure glycerol substrates or require expensive vitamin B12 supplementation, which considerably restricts their economic scalability. To overcome these constraints, a previously isolated Klebsiella pneumoniae strain was engineered to convert crude glycerol to 1,3-propanediol efficiently via adaptive laboratory evolution and rational metabolic engineering. In addition, an adenosine-driven ATP regeneration system was introduced; the engineered strain K. pneumoniae CG6.6 was capable of producing 110 g/L 1,3-propanediol in 48 h in a 5-L bioreactor, achieving a yield of 0.48 g/g without vitamin B12 supplementation. These findings highlight the potential of the engineered K. pneumoniae for sustainable and cost-effective 1,3-propanediol biosynthesis from crude glycerol.