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<p>As a naturally occurring substance derived from plants, β-farnesene represents a significant volatile sesquiterpene with potential applications in the fields of pest control and pharmaceutical intermediates. Nevertheless, the trace and transient production of farnesene from plants imposes limitations on its utilisation. Cyanobacteria, the sole oxygenic photosynthetic bacteria, are optimal chassis cells for farnesene synthesis from CO<sub>2</sub>. In order to achieve efficient and sustained release of β-farnesene, a synthetic pathway based on the endogenous methylerythritol phosphate (MEP) pathway was designed and created in a fast-growing <i>Synechocystis</i> sp. PCC 6803, which is high-light-tolerant and was recently found (named as HL6803). The β-farnesene synthase gene (<i>AaFS</i>) and isopentenyl diphosphate isomerase gene (<i>AaIDI</i>) from <i>Artemisia annua</i> were introduced into the cyanobacterial strain HL6803 for β-farnesene production. A combination of basic engineering strategies resulted in a β-farnesene productivity of up to 2.0 ± 0.4&#xa0;mg/L/day. This is the highest productivity reported using similar engineering strategies. This work contributes to the engineering cyanobacteria for farnesene production from CO<sub>2</sub>, as well as providing a novel fast-growing <i>Synechocystis</i> strain for the production of useful chemicals from CO<sub>2</sub>.</p>

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Metabolic engineering of a high-light tolerant cyanobacterium Synechocystis sp. PCC 6803 for efficient biosynthesis of β-farnesene from CO2

  • 期刊论文

  • 发表日期：

    2025年8月22日

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  • Wei Zhang,
  • Jie Zhou,
  • Yanping Zhang,
  • Yin Li

摘要

As a naturally occurring substance derived from plants, β-farnesene represents a significant volatile sesquiterpene with potential applications in the fields of pest control and pharmaceutical intermediates. Nevertheless, the trace and transient production of farnesene from plants imposes limitations on its utilisation. Cyanobacteria, the sole oxygenic photosynthetic bacteria, are optimal chassis cells for farnesene synthesis from CO2. In order to achieve efficient and sustained release of β-farnesene, a synthetic pathway based on the endogenous methylerythritol phosphate (MEP) pathway was designed and created in a fast-growing Synechocystis sp. PCC 6803, which is high-light-tolerant and was recently found (named as HL6803). The β-farnesene synthase gene (AaFS) and isopentenyl diphosphate isomerase gene (AaIDI) from Artemisia annua were introduced into the cyanobacterial strain HL6803 for β-farnesene production. A combination of basic engineering strategies resulted in a β-farnesene productivity of up to 2.0 ± 0.4 mg/L/day. This is the highest productivity reported using similar engineering strategies. This work contributes to the engineering cyanobacteria for farnesene production from CO2, as well as providing a novel fast-growing Synechocystis strain for the production of useful chemicals from CO2.