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<p>Yeast single cell protein (SCP) is a nutritious protein supplement of artificial feed and food. It is expected that yeast cells grow on nonfood feedstocks instead of agricultural sugars for synthesizing high-quality proteins. Herein, the protein content and quality of the edible yeast <i>Candida utilis</i> were investigated on utilizing ability of carbon and nitrogen sources. We found that dihydroxyacetone (DHA), a feedstock that can be chemically or enzymatically generated from one-carbon (C1) compounds such as methane, methanol and even CO<sub>2</sub>, was comparable with glucose but superior to acetate for <i>C. utilis</i> protein production. The essential amino acid score (EAAS) of DHA-cultured <i>C. utilis</i> protein not only met FAO/WHO (2013) standard, but also surpassed that of benchmark soybean and fish feed. Fed-batch fermentation of <i>C. utilis</i> utilizing DHA feedstock in a 5&#xa0;L fermenter performed a growth rate of 1.3&#xa0;g DCW L<sup>−1</sup> h<sup>−1</sup> and a total of 34.8&#xa0;g L<sup>−1</sup> biomass with the protein content of 60.1% DW, validating scale-up production. This work highlights that <i>C. utilis</i> SCP derived from low-carbon source is a high-quality protein for advancing sustainable feed and food supply.</p>

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Synthesis of single cell protein from C1-derived dihydroxyacetone by Candida utilis

  • 期刊论文

  • 发表日期：

    2025年2月20日

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  • Yajing Zhang,
  • Xupeng Cao,
  • Wangyin Wang,
  • Can Li

摘要

Yeast single cell protein (SCP) is a nutritious protein supplement of artificial feed and food. It is expected that yeast cells grow on nonfood feedstocks instead of agricultural sugars for synthesizing high-quality proteins. Herein, the protein content and quality of the edible yeast Candida utilis were investigated on utilizing ability of carbon and nitrogen sources. We found that dihydroxyacetone (DHA), a feedstock that can be chemically or enzymatically generated from one-carbon (C1) compounds such as methane, methanol and even CO2, was comparable with glucose but superior to acetate for C. utilis protein production. The essential amino acid score (EAAS) of DHA-cultured C. utilis protein not only met FAO/WHO (2013) standard, but also surpassed that of benchmark soybean and fish feed. Fed-batch fermentation of C. utilis utilizing DHA feedstock in a 5 L fermenter performed a growth rate of 1.3 g DCW L−1 h−1 and a total of 34.8 g L−1 biomass with the protein content of 60.1% DW, validating scale-up production. This work highlights that C. utilis SCP derived from low-carbon source is a high-quality protein for advancing sustainable feed and food supply.