<p>The record of unusual prey in opportunistic predation events can reveal the dietary flexibility of some aquatic bird species and contribute to the identification of potential prey present in the environment. This study presents the first recorded predation of a golden tegu lizard (<i>Tupinambis teguixin</i>) by the rufescent tiger-heron (<i>Tigrisoma lineatum</i>). The event occurred near a black caiman (<i>Melanosuchus niger</i>) nest that was being monitored by camera traps, allowing for a complete photographic record of the predation. The predation lasted approximately eight minutes and may have been influenced by the lizard’s exposure, as it was unable to access the black caiman nest, which was protected by a wire mesh installed to prevent predation during the nest monitoring period at the Mamirauá Sustainable Development Reserve, Amazonas, Brazil. The golden tegu lizard (<i>Tupinambis teguixin</i>) is one of the main predators of black caiman (<i>Melanosuchus niger</i>) eggs, responsible for up to 46% of egg predation in this species’ nests in várzea environments in Central Amazonia. Although predation of lizards by the rufescent tiger heron is uncommon, this interaction may occur more frequently than previously thought in Central Amazonia. This record expands knowledge on the trophic ecology of the rufescent tiger-heron and adds a new predator to the golden tegu, one of the main consumers of black caiman eggs in the Amazon.</p>