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<p>The growing demand for sustainable biopolymers drives the search for efficient microbial exopolysaccharide (EPS) producers capable of utilizing low-cost substrates. In this study, we isolated and characterized novel EPS-producing strains from agro-industrial and oil-contaminated waste in the Republic of Tatarstan, Russia. Four selected isolates were identified as <i>Bacillus paramycoides</i>, <i>Pantoea vagans</i>, <i>Bacillus vallismortis</i>, and <i>Bacillus albus</i> based on 16 S rRNA gene sequencing (≥ 99% similarity). Using sugar beet molasses as a primary carbon source, we systematically optimized the carbon-to-nitrogen (C/N) ratio in both minimal (MSM) and rich (Banik) media. The highest EPS yield of 21.11 g l<sup>− 1</sup> was achieved by <i>B. vallismortis</i> 14wfo in Banik medium with a C/N ratio of 10:1. for most strains, including <i>Xanthomonas arboricola</i> and <i>X. cynarea</i>, the most balanced growth and EPS production was observed in MSM medium with molasses at a C/N ratio of 20:1, yielding up to 10.70 g l<sup>− 1</sup>. FTIR spectroscopy confirmed the polysaccharide nature of the EPS and revealed structural features typical of anionic polymers, including xanthan-like spectra for <i>Xanthomonas</i> spp. Cluster analysis identified three metabolic strategies among the strains: generalists (<i>B. vallismortis</i>, <i>X. arboricola</i>), specialists (<i>X. cynarea</i>, <i>P. vagans</i>, <i>X. campestris</i>), and conservative producers (<i>B. paramycoides</i>, <i>B. albus</i>). This study demonstrates the effective use of regional agro-waste for EPS production and provides a basis for tailoring cultivation conditions to strain-specific metabolism.</p>

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Enhanced exopolysaccharide production by novel strains using optimized agro-waste-based media with a carbon-to-nitrogen ratio

  • 期刊论文

  • 发表日期：

    2026年7月11日

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  • Anastasiia Kirichenko,
  • Liliya Biktasheva,
  • Alexander Gordeev,
  • Adelina Kabirova,
  • Polina Kuryntseva

摘要

The growing demand for sustainable biopolymers drives the search for efficient microbial exopolysaccharide (EPS) producers capable of utilizing low-cost substrates. In this study, we isolated and characterized novel EPS-producing strains from agro-industrial and oil-contaminated waste in the Republic of Tatarstan, Russia. Four selected isolates were identified as Bacillus paramycoides, Pantoea vagans, Bacillus vallismortis, and Bacillus albus based on 16 S rRNA gene sequencing (≥ 99% similarity). Using sugar beet molasses as a primary carbon source, we systematically optimized the carbon-to-nitrogen (C/N) ratio in both minimal (MSM) and rich (Banik) media. The highest EPS yield of 21.11 g l− 1 was achieved by B. vallismortis 14wfo in Banik medium with a C/N ratio of 10:1. for most strains, including Xanthomonas arboricola and X. cynarea, the most balanced growth and EPS production was observed in MSM medium with molasses at a C/N ratio of 20:1, yielding up to 10.70 g l− 1. FTIR spectroscopy confirmed the polysaccharide nature of the EPS and revealed structural features typical of anionic polymers, including xanthan-like spectra for Xanthomonas spp. Cluster analysis identified three metabolic strategies among the strains: generalists (B. vallismortis, X. arboricola), specialists (X. cynarea, P. vagans, X. campestris), and conservative producers (B. paramycoides, B. albus). This study demonstrates the effective use of regional agro-waste for EPS production and provides a basis for tailoring cultivation conditions to strain-specific metabolism.