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<p>The bioplastic Poly (3-hydroxybutyrate) (PHB) obtained from Gram positive bacteria has been reported to be superior to the commercially available PHB. A mathematical model of batch culture of <i>Bacillus subtilis</i> was extrapolated to predict fed-batch cultivation strategies. Four different fed-batch studies: pseudo steady state with respect to substrate, constant feed rate, increasing and decreasing feed rate strategies were performed under shake flask conditions. A PHB productivity of 0.046&#xa0;g/l/h and PHB yield of 71.73% of dry cell weight was obtained using the constant feed rate strategy. The PHB extraction process was also optimized using statistical tools. Four extraction variables: solvent pH, solvent extraction temperature, incubation time and solvent percentage were studied. The percentage of solvent used affected the PHB yield significantly, whereas, temperature and the period of incubation affected the purity of extracted PHB from the bacterial cell pellet. Maximum predicted PHB yield (0.845&#xa0;g/g) and PHB purity (98.60%) could be obtained by solvent extraction at 43&#xa0;°C for 70&#xa0;minutes.</p>

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Enhanced production of Poly (3-hydroxybutyrate) using fed-batch studies and optimized separation process

  • 期刊论文

  • 发表日期：

    2025年9月6日

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  • Jayprakash Yadav,
  • Nivedita Patra

摘要

The bioplastic Poly (3-hydroxybutyrate) (PHB) obtained from Gram positive bacteria has been reported to be superior to the commercially available PHB. A mathematical model of batch culture of Bacillus subtilis was extrapolated to predict fed-batch cultivation strategies. Four different fed-batch studies: pseudo steady state with respect to substrate, constant feed rate, increasing and decreasing feed rate strategies were performed under shake flask conditions. A PHB productivity of 0.046 g/l/h and PHB yield of 71.73% of dry cell weight was obtained using the constant feed rate strategy. The PHB extraction process was also optimized using statistical tools. Four extraction variables: solvent pH, solvent extraction temperature, incubation time and solvent percentage were studied. The percentage of solvent used affected the PHB yield significantly, whereas, temperature and the period of incubation affected the purity of extracted PHB from the bacterial cell pellet. Maximum predicted PHB yield (0.845 g/g) and PHB purity (98.60%) could be obtained by solvent extraction at 43 °C for 70 minutes.