<p>There is now robust evidence that children’s preferences for play mates and toys are influenced by gender stereotypes, but most studies investigating this phenomenon have been conducted with children from the United States. This is the first study to investigate effects of gender stereotyping on Brazilian children’s preferences for animated short-films. Fifty-two preschool aged children participated. Children were assessed by a translated and adapted version of the Short POAT scale (Preschool Occupations, Activities and Traits Scale). They were also interviewed about their favorite movies/films and next, using a paired preference software. Participants were asked to choose one short-film out of nine available (three rated previously as films for “girls”, three for “boys”, and three that were considered neutral). Our results showed that 90.3% of participants showed moderate to high levels of gender stereotyping, but children's self/gender-typed preference scores for activities (e.g., "how much do you like to play with baby dolls?") were significantly higher than their self/gender-typed preference scores for occupations (e.g., "how much would you like to be a nurse"?). No correlation was found between stereotyping level and children’s preferences for the new short-film, but a significant correlation was found between POAT-AM scores and children’s existing preferences for other movies/films. These findings suggest that Brazilian children are still very much influenced by gender stereotypes, however, when given an opportunity to choose freely between different short films, their preferences seem to be more driven by their own interests, rather than by a gender bias.</p>