<p>Infertility is an important health concern that affects around 15% of couples, 40–50% of infertile cases are because of malefactors. Azoospermia is known as one of the important causes of male infertility. PRP is an autologous source of growth factors used in various therapeutic strategies. In the present study, PRP was injected into mice scrotum, after induced azoospermia caused by scrotal hyperthermia, and then therapeutic effects were evaluated. 24 adult male mice were divided into 4 groups: Control, Azoospermia (model induced by scrotal hyperthermia every other day after anesthesia for 35 days), and ketamine/xylazine (Ket/Xi) (to assess the probable effect of anesthesia), PRP (injected 10ul of PRP in the scrotum of azoospermia mice) after 16 days animals were anesthetized and sacrificed. Plasma testosterone, seminiferous diameter, oxidative stress, and sperm parameters were evaluated. Plasma testosterone level in the Azoospermia group significantly decreased and PRP treatment improved it. Also, the testicular tissue showed impairment, and oxidative stress levels increased in the testes in the Azoospermia group and PRP treatment ameliorated them. Spermatogenesis completely arrested after scrotal hyperthermia that after treatment with PRP, resumed. PRP injection in the scrotum resumed spermatogenesis and increased the production of testosterone, reduced the oxidative stress level in the testicular tissue, and resumed sperm production. PRP shows promise in promoting testicular recovery following hyperthermia-induced damage.</p> Graphical Abstract <p></p>