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<p>Karyotypic data on the world’s smallest mammal—the Etruscan shrew <i>Suncus etruscus</i>—and an endemic of the Siculo-Maltese archipelago—the Sicilian shrew <i>Crocidura sicula</i>—have so far been limited to a few conventional and differentially stained karyograms, respectively. We present the first detailed characterization of karyotypes of these species using several differential staining (G- and C-banding) and advanced molecular cytogenetic techniques (CMA3-DAPI and FISH of telomeric and 5.8S/18S/28S ribosomal-DNA probes). The <i>S. etruscus</i> female karyotype was found to have 2n = 42, NF = 78; differential staining was applied for the first time. C-positive heterochromatic blocks were detected in centromeric regions of all autosomes (except for six C-negative pairs) and&#xa0;terminally on autosome pair 14. X chromosomes are C-negative. Karyotypes of <i>C. sicula</i> were found to have 2n = 36, NF = 56. G-banding was applied for the first time to specimens originating from Sicily Island and appeared very similar to the only available G-karyogram from Malta Island (where the species is considered extinct). The examined <i>C. sicula</i> karyotypes differ from some previously published ones in chromosome morphology and the number and localization of C-blocks. By CMA3-DAPI after G-banding, we showed that the <i>S. etruscus</i> karyotype contains less GC-rich heterochromatin as compared to the <i>C. sicula</i> karyotype. In both examined species, FISH signals of telomeric DNA were detectable at terminal loci of all chromosomes; interstitial signals were absent. Clusters of ribosomal DNA were observed at a terminal position in two and six autosome pairs in <i>S. etruscus</i> and <i>C. sicula</i> karyotypes, respectively. </p>

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Detailed characterization of karyotypes of white-toothed shrews Suncus etruscus and Crocidura sicula (Eulipotyphla, Mammalia)

  • 期刊论文

  • 发表日期：

    2025年5月28日

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  • Svetlana V. Pavlova,
  • Larisa S. Biltueva,
  • Natalya A. Lemskaya,
  • Natalya A. Serdyukova

摘要

Karyotypic data on the world’s smallest mammal—the Etruscan shrew Suncus etruscus—and an endemic of the Siculo-Maltese archipelago—the Sicilian shrew Crocidura sicula—have so far been limited to a few conventional and differentially stained karyograms, respectively. We present the first detailed characterization of karyotypes of these species using several differential staining (G- and C-banding) and advanced molecular cytogenetic techniques (CMA3-DAPI and FISH of telomeric and 5.8S/18S/28S ribosomal-DNA probes). The S. etruscus female karyotype was found to have 2n = 42, NF = 78; differential staining was applied for the first time. C-positive heterochromatic blocks were detected in centromeric regions of all autosomes (except for six C-negative pairs) and terminally on autosome pair 14. X chromosomes are C-negative. Karyotypes of C. sicula were found to have 2n = 36, NF = 56. G-banding was applied for the first time to specimens originating from Sicily Island and appeared very similar to the only available G-karyogram from Malta Island (where the species is considered extinct). The examined C. sicula karyotypes differ from some previously published ones in chromosome morphology and the number and localization of C-blocks. By CMA3-DAPI after G-banding, we showed that the S. etruscus karyotype contains less GC-rich heterochromatin as compared to the C. sicula karyotype. In both examined species, FISH signals of telomeric DNA were detectable at terminal loci of all chromosomes; interstitial signals were absent. Clusters of ribosomal DNA were observed at a terminal position in two and six autosome pairs in S. etruscus and C. sicula karyotypes, respectively.