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<p>The global impact of climate change on agricultural production poses a significant threat to rice (<i>Oryza sativa</i>) cultivation which in turn adversely influences the food supply. A systematic approach integrating physiological, biochemical and molecular evaluation is imperative to determine the drought tolerant potential of unexplored traditional rice landraces. The present study, aims at evaluating the drought response of 26 rice landraces at the reproductive stage, along with checks, Apo (tolerant) and IR64 (susceptible) in rain out shelter. Comprehensive analysis involving 14 morphological, physiological and biochemical parameters, revealed significant variations in traits crucial for drought resilience. Based on the field screening results involving the physio-biochemical analyses, four landraces <i>viz.,</i> IC115439, IC98878, IC115183 and IC124385 were identified as drought tolerant. Under water deficit conditions (stress), these tolerant genotypes exhibited elevated levels of peroxidase and proline, and a decrease in malondialdehyde (MDA) compared to Apo. Furthermore, these tolerant landraces were validated by gene expression analysis using differentially expressed drought linked upregulated genes<i>, </i><i>OsPOX, OsP5CS</i> and <i>OsXTH</i> by quantifying their expression levels. Gene expression analysis revealed that the mechanism of drought tolerance in these genotypes could be attributed to the combination of adaptive strategies by increased activity of peroxidase, proline and <i>XTH</i>. Holistic approach integrating both field screening and molecular analysis resulted in the identification of four drought tolerant landraces (IC115439, IC98878, IC115183 and IC124385). These landraces can be further subjected to large scale testing at multilocation to assess their stability for higher yield and drought tolerance ability.</p>

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Integrated profiling of drought tolerant rice landraces through comprehensive examination of gene expression, morpho-physiological traits, and biochemical responses during reproductive stage

  • 期刊论文

  • 发表日期：

    2026年3月23日

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  • P. Dharani,
  • T. Nivethitha,
  • S. Manju Devi,
  • A. John Joel

摘要

The global impact of climate change on agricultural production poses a significant threat to rice (Oryza sativa) cultivation which in turn adversely influences the food supply. A systematic approach integrating physiological, biochemical and molecular evaluation is imperative to determine the drought tolerant potential of unexplored traditional rice landraces. The present study, aims at evaluating the drought response of 26 rice landraces at the reproductive stage, along with checks, Apo (tolerant) and IR64 (susceptible) in rain out shelter. Comprehensive analysis involving 14 morphological, physiological and biochemical parameters, revealed significant variations in traits crucial for drought resilience. Based on the field screening results involving the physio-biochemical analyses, four landraces viz., IC115439, IC98878, IC115183 and IC124385 were identified as drought tolerant. Under water deficit conditions (stress), these tolerant genotypes exhibited elevated levels of peroxidase and proline, and a decrease in malondialdehyde (MDA) compared to Apo. Furthermore, these tolerant landraces were validated by gene expression analysis using differentially expressed drought linked upregulated genes, OsPOX, OsP5CS and OsXTH by quantifying their expression levels. Gene expression analysis revealed that the mechanism of drought tolerance in these genotypes could be attributed to the combination of adaptive strategies by increased activity of peroxidase, proline and XTH. Holistic approach integrating both field screening and molecular analysis resulted in the identification of four drought tolerant landraces (IC115439, IC98878, IC115183 and IC124385). These landraces can be further subjected to large scale testing at multilocation to assess their stability for higher yield and drought tolerance ability.