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<p>Hybrid&#xa0;rice technology, particularly involving cytoplasmic male sterility (CMS), is critical for increasing rice productivity. The restorer of fertility (Rf) genes plays a crucial role in the successful application of hybrid rice by restoring the fertility CMS line. This study aimed to identify genomic regions and candidate genes associated with fertility restoration in Giza178, an elite Egyptian rice cultivar known for its salinity tolerance and high yielding. An F₂ mapping population was derived from cross between IR58025A (CMS line) and Giza178 (Rf line). Quantitative trait loci (QTL) mapping revealed multiple significant loci for fertility traits, predominantly on chromosome 10. Three major QTLs for pollen fertility, co-localized within the interval surrounding the SRR markers RM171 and RM258, explained substantial portions of the phenotypic variance. Genomic mining within these QTL regions identified several candidate restorer genes, including previously characterized pentatricopeptide repeat (PPR) genes such as <i>Rf4</i>, <i>Rf1</i>, and <i>RF1b</i>. The identified candidate genes and genomic regions offer valuable molecular tools for marker-assisted breeding and represent promising targets for functional validation to facilitate the development of robust hybrid rice varieties suitable for cultivation under Salinity conditions and similar stress-prone regions.</p>

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Molecular characterization of fertility restoration loci in the high-yielding rice cultivar Giza178

  • 期刊论文

  • 发表日期：

    2026年1月8日

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  • Raafat El-Namaky,
  • Saied Soltan,
  • Mohamed Abdelrahman,
  • Nana Kofi

摘要

Hybrid rice technology, particularly involving cytoplasmic male sterility (CMS), is critical for increasing rice productivity. The restorer of fertility (Rf) genes plays a crucial role in the successful application of hybrid rice by restoring the fertility CMS line. This study aimed to identify genomic regions and candidate genes associated with fertility restoration in Giza178, an elite Egyptian rice cultivar known for its salinity tolerance and high yielding. An F₂ mapping population was derived from cross between IR58025A (CMS line) and Giza178 (Rf line). Quantitative trait loci (QTL) mapping revealed multiple significant loci for fertility traits, predominantly on chromosome 10. Three major QTLs for pollen fertility, co-localized within the interval surrounding the SRR markers RM171 and RM258, explained substantial portions of the phenotypic variance. Genomic mining within these QTL regions identified several candidate restorer genes, including previously characterized pentatricopeptide repeat (PPR) genes such as Rf4, Rf1, and RF1b. The identified candidate genes and genomic regions offer valuable molecular tools for marker-assisted breeding and represent promising targets for functional validation to facilitate the development of robust hybrid rice varieties suitable for cultivation under Salinity conditions and similar stress-prone regions.