<p>Glycosyltransferases (GTs) transfer sugars to hormones, lipids, proteins, and specialized metabolites, shaping signaling and metabolic resilience in plants. We present a genome-wide characterization of wheat (<i>Triticum aestivum</i> L.) GTs and their transcriptional dynamics under drought, salinity, and heat. A comparative framework spanning grasses and Arabidopsis resolved four deep clades with strong support and revealed Triticeae-biased expansions. A wheat-focused phylogeny further partitioned TaGTs into five well-supported subfamilies, indicating conserved lineages alongside recent duplications. Multi-layer annotation showed broad subcellular distributions enriched at the plasma membrane, nucleus, endoplasmic reticulum, and Golgi, consistent with signaling and cell-wall glycosylation roles. Gene structures exhibited diverse exon–intron organizations that track phylogenetic groupings, indicating clade-level structural conservation with lineage-specific remodeling. Conserved protein motifs defined a shared catalytic core, whereas clade-specific motifs suggest functional specialization. Promoter analysis of 2-kb upstream regions uncovered abundant hormone-responsive (ABRE, GARE/P-box, AuxRR/TGA, TGACG/CGTCA, TCA) and stress-responsive elements (MBS, LTR, ARE), together with light-responsive motifs, supporting multifactorial regulation. Gene Ontology enrichment pointed to glycosylation/mannosylation, Golgi localization, and cell-wall organization, and a STRING network highlighted putative GT hubs connecting much of the family. Time-course expression profiling revealed stress-specific programs: <i>TaGT1/TaGT2</i> were induced by drought and salinity, <i>TaGT3</i> was strongly heat responsive, and <i>TaGT4/TaGT7</i> showed limited inducibility. This evolutionarily structured and functionally diversified <i>TaGT</i> repertoire yields prioritized, stress-responsive candidates and a systems framework that can be leveraged in breeding and genome editing to accelerate development of climate-resilient wheat.</p>