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<p>The work reported here investigates the effects of temperature on starch hydrolysis carried out by limit dextrinase (LD) and α-glucosidase (AGL) in diverse barley germplasm. The original activity of both the carbohydrases in barley genotypes declined after 10&#xa0;min of heat treatment at 55&#xa0;°C. The decline in thermostability was less than 50% for LD at 55&#xa0;°C, while it was more than 50% of the original activity for AGL. This indicates the higher temperature optima (<i>T</i><sub><i>50</i></sub>) for LD than AGL. We identified five genotypes for LD which had % remaining activity of more than 50% after heat treatment at 55&#xa0;°C and six genotypes for AGL activity. One mutant, BL2096 was found to have high activity for both the studied enzymes. The identified genotypes with high LD and AGL activities and thermostability can be used profitably to find alleles or genetic factors encoding more thermostable enzymes in barley germplasm.</p>

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Identification of barley genotypes for heat stable variant of limit dextrinase and α-glucosidase

  • 期刊论文

  • 发表日期：

    2025年4月25日

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  • Heena Rani,
  • Rachana D. Bhardwaj,
  • Simarjit Kaur,
  • Satvir Kaur Grewal

摘要

The work reported here investigates the effects of temperature on starch hydrolysis carried out by limit dextrinase (LD) and α-glucosidase (AGL) in diverse barley germplasm. The original activity of both the carbohydrases in barley genotypes declined after 10 min of heat treatment at 55 °C. The decline in thermostability was less than 50% for LD at 55 °C, while it was more than 50% of the original activity for AGL. This indicates the higher temperature optima (T50) for LD than AGL. We identified five genotypes for LD which had % remaining activity of more than 50% after heat treatment at 55 °C and six genotypes for AGL activity. One mutant, BL2096 was found to have high activity for both the studied enzymes. The identified genotypes with high LD and AGL activities and thermostability can be used profitably to find alleles or genetic factors encoding more thermostable enzymes in barley germplasm.