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<p>DH technology offers an array of advantages in maize genetics and breeding. This investigation explored the use of purple pigmentation at the center of endosperm as a reliable marker for selecting haploid seeds. The visual marker simplifies the identification of haploid seeds, essential for producing DH plants with high germination rates and successful transition to field conditions. In a randomly mated BC<sub>1</sub>F<sub>1</sub> maize population of LM13/LM14 x Teosinte cross with CIM2GTAIL, this investigation evaluated the effectiveness of the <i>R1-nj</i> (<i>Navajo</i>) marker in distinguishing haploid seeds with center pigmentation showing significant results of germination rates ranged from 64.65% and&#xa0; 55.81%, seedling length ranged from 33 and 34&#xa0;cm, and seedling vigor index ranged from 2002 and 3507 respective population. In field establishment, the LM13 x Teosinte cross produced 48 haploid plants, while the LM14 x Teosinte cross produced 55 haploid plants. Haploid seeds with center endosperm pigmentation exhibited superior performance, demonstrating higher germination rates and better field establishment. The simplicity and cost-effectiveness of the method increase the efficiency of DH technology and accelerate the breeding cycle. Future research will validate its applicability to different maize genotypes and investigate the underlying molecular mechanisms. These results highlighted the potential of this method to optimize haploid induction and selection, thereby contributing to high-yield, resilient maize varieties.</p>

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Leveraging center endosperm pigmentation for haploid seed selection in maize

  • 期刊论文

  • 发表日期：

    2025年4月24日

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  • Velmurugan Senthilkumar,
  • Priya Garkoti,
  • Shankarappa Varalakshmi,
  • Thotla Naresh,
  • Narendra Kumar Singh

摘要

DH technology offers an array of advantages in maize genetics and breeding. This investigation explored the use of purple pigmentation at the center of endosperm as a reliable marker for selecting haploid seeds. The visual marker simplifies the identification of haploid seeds, essential for producing DH plants with high germination rates and successful transition to field conditions. In a randomly mated BC1F1 maize population of LM13/LM14 x Teosinte cross with CIM2GTAIL, this investigation evaluated the effectiveness of the R1-nj (Navajo) marker in distinguishing haploid seeds with center pigmentation showing significant results of germination rates ranged from 64.65% and  55.81%, seedling length ranged from 33 and 34 cm, and seedling vigor index ranged from 2002 and 3507 respective population. In field establishment, the LM13 x Teosinte cross produced 48 haploid plants, while the LM14 x Teosinte cross produced 55 haploid plants. Haploid seeds with center endosperm pigmentation exhibited superior performance, demonstrating higher germination rates and better field establishment. The simplicity and cost-effectiveness of the method increase the efficiency of DH technology and accelerate the breeding cycle. Future research will validate its applicability to different maize genotypes and investigate the underlying molecular mechanisms. These results highlighted the potential of this method to optimize haploid induction and selection, thereby contributing to high-yield, resilient maize varieties.