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<p>Stripe rust caused by <i>Puccinia striiformis</i> f.sp. <i>tritici</i> (<i>Pst</i>) is one of the most devastating diseases of wheat. The ability to produce new pathotypes, breakdown of rust resistance genes and spread over longer distances makes the management of stripe rust a very difficult task. Among all the control measures, use of resistant varieties is very economical and environmentally safe strategy. So, in the present study, efforts were made to mine stripe rust resistance genes from set of 376 European winter wheat lines by seedling reaction test (SRT) against four most prevalent pathotypes of <i>Puccinia striiformis</i> (238S119, 110S119, 110S84 and 46S119) and field evaluation at two different locations Ludhiana (2021–2022, 2022–2023) and Gurdaspur (2022–2023). Seedling response based on Infection type (IT) against each pathotype was categorized into resistant (IT &lt; 3) and susceptible (IT &gt; 3). Out of 374 (germinated) germplasm lines; 258 showed resistance response and 4 showed susceptible response against all the test pathotypes of <i>Pst</i>, 112 lines showed variable responses in terms of virulence to only one, two and three pathotypes combinations. Based on AUDPC (area under disease progress curve) and FRS (final rust severity), 9 showed highly resistant response with AUDPC and FRS = 0 at both the locations against stripe rust. Gene postulation was done by gene-matching technique. Four <i>Yr</i> genes (<i>Yr5</i>, <i>Yr10</i>, <i>Yr15</i>, <i>Yr24</i>/<i>Yr26</i>) were found effective against the test pathotypes. So, closely linked SSR and KASP markers for these genes were used to confirm the presence of these genes. Based on race specific phenotyping IT (&lt; 2), field evaluation (AUDPC &lt; 100) and marker data the presence of <i>Yr5</i>, <i>Yr10</i>, <i>Yr15</i>, <i>Yr24</i>/<i>Yr26</i> were detected singly or in combination.</p>

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Appraisal of European winter wheat against Puccinia striiformis f.sp tritici pathotypes prevalent under North Indian conditions

  • 期刊论文

  • 发表日期：

    2025年2月13日

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  • Mohit Preet Kaur,
  • Jaspal Kaur,
  • Ritu Bala,
  • Puja Srivastava,
  • Achla Sharma,
  • Satinder Kaur,
  • Harmandeep Kaur

摘要

Stripe rust caused by Puccinia striiformis f.sp. tritici (Pst) is one of the most devastating diseases of wheat. The ability to produce new pathotypes, breakdown of rust resistance genes and spread over longer distances makes the management of stripe rust a very difficult task. Among all the control measures, use of resistant varieties is very economical and environmentally safe strategy. So, in the present study, efforts were made to mine stripe rust resistance genes from set of 376 European winter wheat lines by seedling reaction test (SRT) against four most prevalent pathotypes of Puccinia striiformis (238S119, 110S119, 110S84 and 46S119) and field evaluation at two different locations Ludhiana (2021–2022, 2022–2023) and Gurdaspur (2022–2023). Seedling response based on Infection type (IT) against each pathotype was categorized into resistant (IT < 3) and susceptible (IT > 3). Out of 374 (germinated) germplasm lines; 258 showed resistance response and 4 showed susceptible response against all the test pathotypes of Pst, 112 lines showed variable responses in terms of virulence to only one, two and three pathotypes combinations. Based on AUDPC (area under disease progress curve) and FRS (final rust severity), 9 showed highly resistant response with AUDPC and FRS = 0 at both the locations against stripe rust. Gene postulation was done by gene-matching technique. Four Yr genes (Yr5, Yr10, Yr15, Yr24/Yr26) were found effective against the test pathotypes. So, closely linked SSR and KASP markers for these genes were used to confirm the presence of these genes. Based on race specific phenotyping IT (< 2), field evaluation (AUDPC < 100) and marker data the presence of Yr5, Yr10, Yr15, Yr24/Yr26 were detected singly or in combination.