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<p><i>Pyricularia oryzae</i> is the major pathogen affecting primarily rice and many other graminaceous plants. Cultural, morphological and molecular identification was performed&#xa0;using the internal transcribed spacer (ITS). The pathogen was managed in vitro and in vivo using fungicides. Fungal growth was very slow on potato dextrose agar medium (PDA). Maximum growth and sporulation were observed on rice leaf extract agar (RLEA) at 28–30&#xa0;°C and high relative humidity with alternating light and dark periods for 8 and 16&#xa0;h, respectively. The conidia were biseptate, tricelled, pyriform to obclavate, measuring 17–19&#xa0;μm × 5–7&#xa0;μm, with a rounded base and a tapered tip. All treatments were found to be significantly superior to the control in reducing fungal mycelial growth under in vitro conditions, ranging between 61.38 and 79.44% at low concentrations and 78.33 to 92.78% at higher concentrations of fungicides. Tebuconazole 50% + trifloxystrobin 25% WG, 100 and 200&#xa0;ppm recorded the best treatment compared to others in inhibiting mycelial growth by 79.44% and 92.78%, respectively. The minimum percent disease index (12.12%) and percent disease incidence (58.98%) were recorded for tebuconazole 50% + trifloxystrobin 25% WG. The maximum yield of grain (5973.00&#xa0;kg/ha) and straw (9480.20&#xa0;kg/ha) was found in the tebuconazole 50% + trifloxystrobin 25% WG.</p>

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Efficacy of modern combination fungicides for management of Pyricularia oryzae induced rice blast in Western India

  • 期刊论文

  • 发表日期：

    2025年1月22日

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  • Deep Narayan Mishra,
  • Rakesh Kumar Gangwar,
  • Asiknee Dash

摘要

Pyricularia oryzae is the major pathogen affecting primarily rice and many other graminaceous plants. Cultural, morphological and molecular identification was performed using the internal transcribed spacer (ITS). The pathogen was managed in vitro and in vivo using fungicides. Fungal growth was very slow on potato dextrose agar medium (PDA). Maximum growth and sporulation were observed on rice leaf extract agar (RLEA) at 28–30 °C and high relative humidity with alternating light and dark periods for 8 and 16 h, respectively. The conidia were biseptate, tricelled, pyriform to obclavate, measuring 17–19 μm × 5–7 μm, with a rounded base and a tapered tip. All treatments were found to be significantly superior to the control in reducing fungal mycelial growth under in vitro conditions, ranging between 61.38 and 79.44% at low concentrations and 78.33 to 92.78% at higher concentrations of fungicides. Tebuconazole 50% + trifloxystrobin 25% WG, 100 and 200 ppm recorded the best treatment compared to others in inhibiting mycelial growth by 79.44% and 92.78%, respectively. The minimum percent disease index (12.12%) and percent disease incidence (58.98%) were recorded for tebuconazole 50% + trifloxystrobin 25% WG. The maximum yield of grain (5973.00 kg/ha) and straw (9480.20 kg/ha) was found in the tebuconazole 50% + trifloxystrobin 25% WG.