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<p><UnorderedList Mark="Bullet"> <ItemContent> <p>Both tomato cultivar identity and Ralstonia solanacearum pathogen invasion distinctly reshape rhizosphere bacterial and protistan communities.</p> </ItemContent> <ItemContent> <p><i>R. solanacearum</i> pathogen infection primarily alters bacterial community composition, while tomato cultivar identity exerts a stronger effect on protists.</p> </ItemContent> <ItemContent> <p><i>R. solanacearum</i> pathogen invasion shifts bacterial community assembly from deterministic toward stochastic processes while protistan assembly remains predominantly stochastic.</p> </ItemContent> </UnorderedList></p><p>Bacterial wilt, caused by <i>Ralstonia solanacearum</i> (RS), is a major constraint on tomato production worldwide. This study investigated how multitrophic rhizosphere communities associated with five tomato cultivars (AM, DF, MF, NP, and ZY) respond to RS invasion using sterile plate and greenhouse pot assays. Rhizosphere bacterial and protistan communities were characterized by 16S and 18S rRNA gene amplicon sequencing under control and RS-inoculated conditions. Results showed that both cultivar identity and RS invasion significantly shaped microbial structures and compositions. RS infection exerted a stronger influence on bacterial communities than protists, whereas cultivar identity was the primary driver for protistan shifts. Random forest classification and differential abundance analysis identified specific microbial taxa that effectively discriminated cultivars and infection status. Community assembly analysis further revealed that bacterial assembly shifted from predominantly deterministic to more stochastic processes under pathogen pressure, while protistan assembly remained largely stochastic. Collectively, these results demonstrate that tomato cultivars harbor distinct multi-trophic rhizosphere microbial configurations under pathogen invasion, and highlight the importance of incorporating protists into rhizosphere community frameworks for understanding and potentially enhancing bacterial wilt suppression.</p>

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Cultivar-dependent responses of tomato rhizosphere bacterial and protistan communities under Ralstonia solanacearum invasion

  • 期刊论文

  • 发表日期：

    2026年7月18日

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  • Jingchi Liu,
  • Yi Ren,
  • Zhipeng Zhao,
  • Shiyu Shang,
  • Kanglong Zhou,
  • Lang Zhao,
  • Zhiyang Zhang,
  • Yangyi Zhang,
  • Yue Sun,
  • Ruoyan Wang,
  • Xirui Wang,
  • Yang Yue,
  • Chen Liu,
  • Qirong Shen,
  • Wu Xiong

摘要

Both tomato cultivar identity and Ralstonia solanacearum pathogen invasion distinctly reshape rhizosphere bacterial and protistan communities.

R. solanacearum pathogen infection primarily alters bacterial community composition, while tomato cultivar identity exerts a stronger effect on protists.

R. solanacearum pathogen invasion shifts bacterial community assembly from deterministic toward stochastic processes while protistan assembly remains predominantly stochastic.

Bacterial wilt, caused by Ralstonia solanacearum (RS), is a major constraint on tomato production worldwide. This study investigated how multitrophic rhizosphere communities associated with five tomato cultivars (AM, DF, MF, NP, and ZY) respond to RS invasion using sterile plate and greenhouse pot assays. Rhizosphere bacterial and protistan communities were characterized by 16S and 18S rRNA gene amplicon sequencing under control and RS-inoculated conditions. Results showed that both cultivar identity and RS invasion significantly shaped microbial structures and compositions. RS infection exerted a stronger influence on bacterial communities than protists, whereas cultivar identity was the primary driver for protistan shifts. Random forest classification and differential abundance analysis identified specific microbial taxa that effectively discriminated cultivars and infection status. Community assembly analysis further revealed that bacterial assembly shifted from predominantly deterministic to more stochastic processes under pathogen pressure, while protistan assembly remained largely stochastic. Collectively, these results demonstrate that tomato cultivars harbor distinct multi-trophic rhizosphere microbial configurations under pathogen invasion, and highlight the importance of incorporating protists into rhizosphere community frameworks for understanding and potentially enhancing bacterial wilt suppression.